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Article

Differential Physiological Prerequisites and Gene Expression Profiles of Conidial Anastomosis Tube and Germ Tube Formation in Colletotrichum gloeosporioides

1
National Fungal Culture Collection of India (NFCCI), Biodiversity and Palaeobiology Group, MACS-Agharkar Research Institute, G.G. Agarkar Road, Pune 411004, India
2
Department of Microbiology, Savitribai Phule Pune University, Pune 411007, India
3
Genetics and Plant Breeding Group, MACS-Agharkar Research Institute, G.G. Agarkar Road, Pune 411004, India
*
Author to whom correspondence should be addressed.
Academic Editor: Pedro Gonçalves
J. Fungi 2021, 7(7), 509; https://doi.org/10.3390/jof7070509
Received: 11 June 2021 / Revised: 21 June 2021 / Accepted: 22 June 2021 / Published: 25 June 2021
(This article belongs to the Special Issue Emergent Fungal Models for Genetics and Cell Biology)
The conidia of a hemibiotrophic fungus, Colletotrichum gloeosporioides, can conventionally form a germ tube (GT) and develop into a fungal colony. Under certain conditions, they tend to get connected through a conidial anastomosis tube (CAT) to share the nutrients. CAT fusion is believed to be responsible for the generation of genetic variations in few asexual fungi, which appears problematic for effective fungal disease management. The physiological and molecular requirements underlying the GT formation versus CAT fusion remained underexplored. In the present study, we have deciphered the physiological prerequisites for GT formation versus CAT fusion in C. gloeosporioides. GT formation occurred at a high frequency in the presence of nutrients, while CAT fusion was found to be higher in the absence of nutrients. Younger conidia were found to form GT efficiently, while older conidia preferentially formed CAT. Whole transcriptome analysis of GT and CAT revealed highly differential gene expression profiles, wherein 11,050 and 9786 genes were differentially expressed during GT formation and CAT fusion, respectively. A total of 1567 effector candidates were identified; out of them, 102 and 100 were uniquely expressed during GT formation and CAT fusion, respectively. Genes coding for cell wall degrading enzymes, germination, hyphal growth, host-fungus interaction, and virulence were highly upregulated during GT formation. Meanwhile, genes involved in stress response, cell wall remodeling, membrane transport, cytoskeleton, cell cycle, and cell rescue were highly upregulated during CAT fusion. To conclude, the GT formation and CAT fusion were found to be mutually exclusive processes, requiring differential physiological conditions and sets of DEGs in C. gloeosporioides. This study will help in understanding the basic CAT biology in emerging fungal model species of the genus Colletotrichum. View Full-Text
Keywords: Colletotrichum gloeosporioides; germ tube; conidial anastomosis tube; transcriptome; qPCR; anthracnose; gene annotation Colletotrichum gloeosporioides; germ tube; conidial anastomosis tube; transcriptome; qPCR; anthracnose; gene annotation
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MDPI and ACS Style

Mehta, N.; Patil, R.; Baghela, A. Differential Physiological Prerequisites and Gene Expression Profiles of Conidial Anastomosis Tube and Germ Tube Formation in Colletotrichum gloeosporioides. J. Fungi 2021, 7, 509. https://doi.org/10.3390/jof7070509

AMA Style

Mehta N, Patil R, Baghela A. Differential Physiological Prerequisites and Gene Expression Profiles of Conidial Anastomosis Tube and Germ Tube Formation in Colletotrichum gloeosporioides. Journal of Fungi. 2021; 7(7):509. https://doi.org/10.3390/jof7070509

Chicago/Turabian Style

Mehta, Nikita, Ravindra Patil, and Abhishek Baghela. 2021. "Differential Physiological Prerequisites and Gene Expression Profiles of Conidial Anastomosis Tube and Germ Tube Formation in Colletotrichum gloeosporioides" Journal of Fungi 7, no. 7: 509. https://doi.org/10.3390/jof7070509

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