Deletion of ptn1, a PTEN/TEP1 Orthologue, in Ustilago maydis Reduces Pathogenicity and Teliospore Development
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Growth Conditions
2.2. Vector Construction and Nucleic Acid Manipulations
2.3. RNA Isolation and Expression Analysis
2.4. Cell Growth, Mating, and Plant Infection
2.5. Statistical Analysis
3. Results
3.1. In Silico Analysis of ptn1
3.2. Deletion of ptn1 Led to the Reduced Production of the Aerial Hyphae Characteristics of the Mating Reaction on Charcoal Media
3.3. prf1 Is Upregulated in Δptn1 under Low Ammonium Conditions
3.4. Cell Growth of ptn1 Mutants Is Largely Unaffected, while Stress Tolerance Is Reduced under Certain Conditions
3.5. Pathogenicity Is Reduced by ptn1 Deletion
3.6. Pathogenicity Calculated by Additional Plant Growth Indicators
3.7. Impact of ptn1 Mutations on Spore Formation and Germination
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ustilago Strain | Genotype a | References |
---|---|---|
1/2 | a1b1 | [19] |
2/9 | a2b2 | [19] |
1/2 Δptn1 | a1b1 ptn1::cbxR | This study |
1/2 Δptn1J | a1b1 ptn1::hygR | This study |
2/9 Δptn1 | a2b2 ptn1::cbxR | This study |
2/9 Δptn1J | a2b2 ptn1::hygR | This study |
FB1 Δptn1J | a1b1 ptn1::hygR | This study |
1/2 ptn1otef | a1b1 Potef-ump2, cbxR | This study |
2/9 ptn1otef | a2b2 Potef-ump2, cbxR | This study |
SG200 | a1mfa2 bE1bW2 | [20] |
SG200 Δptn1 | a1mfa2 bE1bW2 ptn1::hygR | This study |
d132 | a1a2b1b2 | [21] |
d132 Δptn1 | a1a2b1b2 ptn1:: hygR | This study |
1/2 Δptn1c | a1b1 ptn1::cbxR Potef-ptn1, hygR | This study |
2/9 Δptn1c | a2b2 ptn1::cbxR Potef-ptn1, hygR | This study |
FBD11-7 | a1a1 b1b2 | [16] |
FBD12-17 | a2a2 b1b2 | [16] |
Plasmids | Genotype | References |
DelsGate ptn1 | kanR ptn1::cbxR | [22] |
Otef-ptn1-cbx | bla(ampR) Potef-ptn1 cbxR | This study |
Otef-ptn1-hyg | bla(ampR) Potef-ptn1 hygR | This study |
Primers | Sequence (5’-3’) |
---|---|
UPTN-K1 | TAGGGATAACAGGGTAATCAACAAAATGACGCAACCAC |
UPTN-K3 | GGGACAAGTTTGTACAAAAAAGCAGGCTAAGAACGATGTTTGCGTGTCAG |
UPTN-K4 | GGGGACCACTTTGTACAAGAAAGCTGGGTACGTTAGACAAAGCGCAATCA |
UPTN-K2 | GGAAGGTGATCGTGAAGGAA |
PTENUPLT | GCAACAAAATGACGCAACC |
PTENUPRT | GTAGTTACCACGTTCGGCCATAAGGTGGTGGGACTGCTTT |
PTENDNLT | GCTTTGCTCTTTGCTCTTCTG |
CPDNRT | GCCTTAATTAATATACGCCCGATTGCTTACC |
HYGPTENLT | AAAGCAGTCCCACCACCTTATGGCCGAACGTGGTAACTAC |
HYGPTENRT | AGAAGAGCAAAGAGCAAAGCCTCAGGCCTCATGTTTGACA |
PTEN5 | TGAGGCCTGAGTGGCCTATATACCCCCTGCCCCTGT |
PTEN3 | CGAATTCTTGCGCTTTGTCTAACGAAC |
HYGSPH1RTNEW | TTTGCATGCCTCAGGCCTCATGTTTGACA |
HYGMFE1 | TTTCAATTGTGGCCGAACGTGGTAACTAC |
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Vijayakrishnapillai, L.M.K.; Desmarais, J.S.; Groeschen, M.N.; Perlin, M.H. Deletion of ptn1, a PTEN/TEP1 Orthologue, in Ustilago maydis Reduces Pathogenicity and Teliospore Development. J. Fungi 2019, 5, 1. https://doi.org/10.3390/jof5010001
Vijayakrishnapillai LMK, Desmarais JS, Groeschen MN, Perlin MH. Deletion of ptn1, a PTEN/TEP1 Orthologue, in Ustilago maydis Reduces Pathogenicity and Teliospore Development. Journal of Fungi. 2019; 5(1):1. https://doi.org/10.3390/jof5010001
Chicago/Turabian StyleVijayakrishnapillai, Lalu M. K., John S. Desmarais, Michael N. Groeschen, and Michael H. Perlin. 2019. "Deletion of ptn1, a PTEN/TEP1 Orthologue, in Ustilago maydis Reduces Pathogenicity and Teliospore Development" Journal of Fungi 5, no. 1: 1. https://doi.org/10.3390/jof5010001