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Volume 11
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10.3390/jof11060453
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Genomic and Functional Analysis of a Novel Yeast Cyberlindnera fabianii TBRC 4498 for High-Yield Xylitol Production

by
Pawarin Bonthong
1,
Benjarat Bunterngsook
2,
Wuttichai Mhuantong
2,
Katesuda Aiewviriyasakul
2,
Wipawee Sritusnee
2,
Verawat Champreda
2 and
Hataikarn Lekakarn
1,*
1
Department of Biotechnology, Faculty of Science and Technology, Rangsit Campus, Thammasat University, Pathum Thani 12120, Thailand
2
Enzyme Technology Research Team, Biorefinery Technology and Bioproduct Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Khlong Luang, Pathum Thani 12120, Thailand
*
Author to whom correspondence should be addressed.
J. Fungi 2025, 11(6), 453; https://doi.org/10.3390/jof11060453 (registering DOI)
Submission received: 11 April 2025 / Revised: 6 May 2025 / Accepted: 11 June 2025 / Published: 13 June 2025
(This article belongs to the Section Fungi in Agriculture and Biotechnology)
Versions Notes

Abstract

The development of yeast cell factories for efficient xylose utilization and xylitol production is crucial for advancing sustainable biotechnological processes. Xylose, a major component of lignocellulosic biomass, presents challenges for microbial conversion due to its complex metabolic pathways. This study presents the genomic perspective and xylitol production capability of a novel xylose utilizing yeast Cyberlindnera fabianii TBRC 4498. Genome sequencing and functional annotation revealed key metabolic networks and genes involved in the xylose metabolism pathway, providing insights into the strain’s performance. The Cy. fabianii TBRC 4498 had excellent growth and xylose assimilation at a broad range of xylose concentrations from 40 to 140 g/L, with the highest growth rate at 80 g/L of xylose. The highest xylitol production yield (83.19 g/L) was detected from 120 g/L of xylose at 30 °C for 72 h, equivalent to 0.65 g xylitol/g xylose and 1.16 g/L/h productivity. Remarkably, Cy. fabianii TBRC 4498 produced high-purity xylitol, achieving over 95% homogeneity without forming undesirable byproducts, such as acid or ethanol. These results demonstrated the potential of Cy. fabianii TBRC 4498 as a whole-cell biocatalyst for xylitol production using high xylose concentrations, offering a promising microbial cell factory for large-scale xylitol production from lignocellulosic sugar.
Keywords: Cyberlindnera fabianii; xylitol; xylose; cell factory; genome Cyberlindnera fabianii; xylitol; xylose; cell factory; genome

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MDPI and ACS Style

Bonthong, P.; Bunterngsook, B.; Mhuantong, W.; Aiewviriyasakul, K.; Sritusnee, W.; Champreda, V.; Lekakarn, H. Genomic and Functional Analysis of a Novel Yeast Cyberlindnera fabianii TBRC 4498 for High-Yield Xylitol Production. J. Fungi 2025, 11, 453. https://doi.org/10.3390/jof11060453

AMA Style

Bonthong P, Bunterngsook B, Mhuantong W, Aiewviriyasakul K, Sritusnee W, Champreda V, Lekakarn H. Genomic and Functional Analysis of a Novel Yeast Cyberlindnera fabianii TBRC 4498 for High-Yield Xylitol Production. Journal of Fungi. 2025; 11(6):453. https://doi.org/10.3390/jof11060453

Chicago/Turabian Style

Bonthong, Pawarin, Benjarat Bunterngsook, Wuttichai Mhuantong, Katesuda Aiewviriyasakul, Wipawee Sritusnee, Verawat Champreda, and Hataikarn Lekakarn. 2025. "Genomic and Functional Analysis of a Novel Yeast Cyberlindnera fabianii TBRC 4498 for High-Yield Xylitol Production" Journal of Fungi 11, no. 6: 453. https://doi.org/10.3390/jof11060453

APA Style

Bonthong, P., Bunterngsook, B., Mhuantong, W., Aiewviriyasakul, K., Sritusnee, W., Champreda, V., & Lekakarn, H. (2025). Genomic and Functional Analysis of a Novel Yeast Cyberlindnera fabianii TBRC 4498 for High-Yield Xylitol Production. Journal of Fungi, 11(6), 453. https://doi.org/10.3390/jof11060453

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