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Veterinary Sciences
Volume 9
Issue 3
10.3390/vetsci9030119
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Article

In Vivo Recovery of Bacteriophages and Their Effects on Clostridium perfringens-Infected Broiler Chickens

by
Hyun-Gwan Lee
1,
Yoo-Bhin Kim
1,
Sang-Hyeok Lee
1,
Jun-Ok Moon
2,
Jong-Pyo Chae
2,
Yu-Jin Kim
2 and
Kyung-Woo Lee
1,*
1
Department of Animal Science and Technology, Konkuk University, Seoul 05029, Korea
2
CJ Cheiljedang Co., Ltd., Suwon 16495, Korea
*
Author to whom correspondence should be addressed.
Vet. Sci. 2022, 9(3), 119; https://doi.org/10.3390/vetsci9030119
Submission received: 19 February 2022 / Revised: 3 March 2022 / Accepted: 4 March 2022 / Published: 7 March 2022
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Abstract

The objectives of this study were to recover bacteriophages (BPs) from the intestinal digesta of BP-fed broilers and to evaluate the antibacterial effects of encapsulated or powdered BPs in broiler chickens challenged with Clostridium perfringens. Day-old broiler chicks (n = 320/experiment) were randomly assigned to 32 pens (n = 10 broilers/pen) and allocated to one of four dietary groups: (1) unchallenged group (NEG); (2) C. perfringens-challenged group (POS); (3) POS group fed a diet supplemented with powdered BPs; and (4) POS group fed a diet supplemented with encapsulated BPs. On days 21, 22, and 23 post-hatch, all chickens except NEG were orally inoculated twice a day with 2 mL C. perfringens (1.0 × 108 cfu/mL). Varying BP levels were detected in gut digesta at all ages and were numerically or significantly higher in the encapsulated BP group than in the powdered BP group. Dietary powder or encapsulated BPs reversed the C. perfringens-mediated increase in crypt depth. In addition, villus height to crypt depth ratio was elevated in the NEG and BP-treated/challenged groups compared with that in the POS group. C. perfringens counts in the cecum were significantly lower in the BP-fed chickens than in the POS group. The encapsulated BP-supplemented diet-fed chickens had the highest serum IgA levels. Collectively, our results suggest that dietary BP remains viable in intestinal digesta upon ingestion and can inhibit cecal C. perfringens counts.
Keywords: Clostridium perfringens; bacteriophage; broiler chicken; gut health Clostridium perfringens; bacteriophage; broiler chicken; gut health

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MDPI and ACS Style

Lee, H.-G.; Kim, Y.-B.; Lee, S.-H.; Moon, J.-O.; Chae, J.-P.; Kim, Y.-J.; Lee, K.-W. In Vivo Recovery of Bacteriophages and Their Effects on Clostridium perfringens-Infected Broiler Chickens. Vet. Sci. 2022, 9, 119. https://doi.org/10.3390/vetsci9030119

AMA Style

Lee H-G, Kim Y-B, Lee S-H, Moon J-O, Chae J-P, Kim Y-J, Lee K-W. In Vivo Recovery of Bacteriophages and Their Effects on Clostridium perfringens-Infected Broiler Chickens. Veterinary Sciences. 2022; 9(3):119. https://doi.org/10.3390/vetsci9030119

Chicago/Turabian Style

Lee, Hyun-Gwan, Yoo-Bhin Kim, Sang-Hyeok Lee, Jun-Ok Moon, Jong-Pyo Chae, Yu-Jin Kim, and Kyung-Woo Lee. 2022. "In Vivo Recovery of Bacteriophages and Their Effects on Clostridium perfringens-Infected Broiler Chickens" Veterinary Sciences 9, no. 3: 119. https://doi.org/10.3390/vetsci9030119

APA Style

Lee, H.-G., Kim, Y.-B., Lee, S.-H., Moon, J.-O., Chae, J.-P., Kim, Y.-J., & Lee, K.-W. (2022). In Vivo Recovery of Bacteriophages and Their Effects on Clostridium perfringens-Infected Broiler Chickens. Veterinary Sciences, 9(3), 119. https://doi.org/10.3390/vetsci9030119

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