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Veterinary Sciences
Volume 7
Issue 3
10.3390/vetsci7030106
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Article

Improvement of the Enterotoxigenic Escherichia coli Infection Model for Post-Weaning Diarrhea by Controlling for Bacterial Adhesion, Pig Breed and MUC4 Genotype

by
Hiroki Matsumoto
1,*,
Masashi Miyagawa
1,
Sayaka Takahashi
2,
Ryouichi Shima
3 and
Takayuki Oosumi
1
1
Research and Development Section, Institute of Animal Health, JA Zen-Noh (National Federation of Agricultural Cooperative Associations), 7 Ohja-machi, Sakura-shi, Chiba 285-0043, Japan
2
Diagnostic Center, Institute of Animal Health, JA Zen-Noh (National Federation of Agricultural Cooperative Associations), 7 Ohja-machi, Sakura-shi, Chiba 285-0043, Japan
3
JA Zen-Noh (National Federation of Agricultural Cooperative Associations), 1-3-1 Ootemachi, Chiyoda-ku, Tokyo 100-6832, Japan
*
Author to whom correspondence should be addressed.
Vet. Sci. 2020, 7(3), 106; https://doi.org/10.3390/vetsci7030106
Submission received: 10 June 2020 / Revised: 29 July 2020 / Accepted: 6 August 2020 / Published: 7 August 2020
(This article belongs to the Section Veterinary Microbiology, Parasitology and Immunology)
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Abstract

Enterotoxigenic Escherichia coli (ETEC) is a major cause of post-weaning diarrhea (PWD) in pigs and causes significant damage to the swine industry worldwide. In recent years, there has been increased regulation against the use of antibacterial agents in swine due to their health risks. Utilizing experimental models that consistently recapitulate PWD is important for the development of non-antibacterial agents against PWD in pigs. In this study, we established a highly reproducible PWD infection model by examining differences in adhesion of ETEC to the intestinal tissue as well as the association between MUC4 polymorphisms and sensitivity to PWD. Post-weaning diarrhea differences between pig breeds were also examined. The adhesion to enterocytes varied from 104.0 to 106.4 CFU/mL even among the F4 ETEC strains. Experimental infection revealed that PWD can be induced in all MUC4 genotypes after infection with 1010 CFU/pig of highly adherent ETEC, although there were variable sensitivities between the genotypes. Lowly adherent ETEC did not cause PWD as efficiently as did highly adherent ETEC. The incidence of PWD was confirmed for all pigs with the ETEC-susceptible MUC4 genotypes in all of the breeds. These results indicate that high-precision and reproducible experimental infection is possible regardless of pig breeds by controlling factors on the pig-end (MUC4 genotype) and the bacterial-end (adhesion ability).
Keywords: enterotoxigenic E. coli; post-weaning diarrhea model; pig; MUC4 genotype; experimental infection enterotoxigenic E. coli; post-weaning diarrhea model; pig; MUC4 genotype; experimental infection

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MDPI and ACS Style

Matsumoto, H.; Miyagawa, M.; Takahashi, S.; Shima, R.; Oosumi, T. Improvement of the Enterotoxigenic Escherichia coli Infection Model for Post-Weaning Diarrhea by Controlling for Bacterial Adhesion, Pig Breed and MUC4 Genotype. Vet. Sci. 2020, 7, 106. https://doi.org/10.3390/vetsci7030106

AMA Style

Matsumoto H, Miyagawa M, Takahashi S, Shima R, Oosumi T. Improvement of the Enterotoxigenic Escherichia coli Infection Model for Post-Weaning Diarrhea by Controlling for Bacterial Adhesion, Pig Breed and MUC4 Genotype. Veterinary Sciences. 2020; 7(3):106. https://doi.org/10.3390/vetsci7030106

Chicago/Turabian Style

Matsumoto, Hiroki, Masashi Miyagawa, Sayaka Takahashi, Ryouichi Shima, and Takayuki Oosumi. 2020. "Improvement of the Enterotoxigenic Escherichia coli Infection Model for Post-Weaning Diarrhea by Controlling for Bacterial Adhesion, Pig Breed and MUC4 Genotype" Veterinary Sciences 7, no. 3: 106. https://doi.org/10.3390/vetsci7030106

APA Style

Matsumoto, H., Miyagawa, M., Takahashi, S., Shima, R., & Oosumi, T. (2020). Improvement of the Enterotoxigenic Escherichia coli Infection Model for Post-Weaning Diarrhea by Controlling for Bacterial Adhesion, Pig Breed and MUC4 Genotype. Veterinary Sciences, 7(3), 106. https://doi.org/10.3390/vetsci7030106

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