Genetically Related Mycobacterium bovis Strains Displayed Differential Intracellular Growth in Bovine Macrophages
by
Alejandro Benítez-Guzmán 1
, Hugo Esquivel-Solís 2, Cecilia Romero-Torres 3, Camila Arriaga-Díaz 3 and José A. Gutiérrez-Pabello 1,*
Alejandro Benítez-Guzmán 1, Hugo Esquivel-Solís 2, Cecilia Romero-Torres 3, Camila Arriaga-Díaz 3 and José A. Gutiérrez-Pabello 1,*
1
Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico
2
Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara 44270, Mexico
3
Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, CENID Microbiología, Ciudad de México 05110, Mexico
*
Author to whom correspondence should be addressed.
Vet. Sci. 2019, 6(4), 81; https://doi.org/10.3390/vetsci6040081
Received: 27 September 2019 / Revised: 14 October 2019 / Accepted: 17 October 2019 / Published: 18 October 2019
(This article belongs to the Special Issue Mycobacterial Diseases in Animals)
Molecular typing of bacterial isolates provides a powerful approach for distinguishing Mycobacterium bovis (M. bovis) genotypes. It is known that M. bovis strain virulence plays a role in prevalence and spread of the disease, suggesting that strain virulence and prevailing genotypes are associated. However, it is not well understood whether strain virulence correlates with particular genotypes. In this study, we assessed the in vitro intracellular growth of 18 M. bovis isolates in bovine macrophages as an indicator of bacterial virulence and sought a relationship with the genotype identified by spoligotyping. We found 14 different spoligotypes—11 were already known and three spoligotypes had never been reported before. We identified 2 clusters that were phylogenetically related, containing 10 and 6 strains, respectively, and 2 orphan strains. Intracellular growth and phagocytic rates of 18 M. bovis strains were heterogeneous. Our results suggest that M. bovis intracellular growth and phagocytosis are independent of the bacterial lineage identified by spoligotyping.
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Keywords:
M. bovis; spoligotype; virulence; intracellular replication; macrophages
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MDPI and ACS Style
Benítez-Guzmán, A.; Esquivel-Solís, H.; Romero-Torres, C.; Arriaga-Díaz, C.; Gutiérrez-Pabello, J.A. Genetically Related Mycobacterium bovis Strains Displayed Differential Intracellular Growth in Bovine Macrophages. Vet. Sci. 2019, 6, 81.
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