Next Article in Journal
Pulse Pressure Variation Can Predict the Hemodynamic Response to Pneumoperitoneum in Dogs: A Retrospective Study
Next Article in Special Issue
Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues
Previous Article in Journal / Special Issue
A Review of Immunotherapeutic Strategies in Canine Malignant Melanoma
Article Menu
Issue 1 (March) cover image

Export Article

Open AccessArticle

Tumor–Microenvironment Interaction: Analysis of Mast Cell Populations in Normal Tissue and Proliferative Disorders of the Canine Prostate

1
Faculty of Veterinary Medicine, University of Teramo, 64100 Teramo, Italy
2
Department of Veterinary Medicine, University of Milan, 20154 Milan, Italy
3
Unità Ospedaliera Complessa, Anatomia patologica, Ospedale G. Mazzini, 64100 Teramo, Italy
4
Veterinary Practitioner, 63812 Montegranaro, Italy
*
Author to whom correspondence should be addressed.
Vet. Sci. 2019, 6(1), 16; https://doi.org/10.3390/vetsci6010016
Received: 4 December 2018 / Revised: 25 January 2019 / Accepted: 7 February 2019 / Published: 13 February 2019
  |  
PDF [1636 KB, uploaded 13 February 2019]
  |  

Abstract

Mast cells (MCs) are involved in angiogenesis, tissue remodeling and immunomodulation in several human and animal tumors, although their exact role is still controversial. Since no information is available in canine prostate carcinoma (PC) and normal prostate tissues, the aims of this study were to evaluate the possible correlations between MC distribution, molecular expression and microvessel density (MVD) in normal prostatic tissue and proliferative disorders of the canine prostate. All samples (6 normal, 15 benign prostate hyperplasia-BPH, 8 PC) were stained with Toluidine Blue and immunohistochemically evaluated for tryptase, c-Kit (CD117) and CD31. Mast cell density (MCD) and MVD were quantified by the hot-spot method. MCD was significantly increased in periglandular/peritumoral areas, when compared with intraglandular/intratumoral areas, in all groups (p = 0.03). C-Kit expression was strongly associated with PC (ρ = 0.75 p = 0.03), whereas positive correlation between tryptase and c-Kit expression (ρ = 0.64 p = 0.01) was observed in periglandular areas of BPH. MVD showed a correlation with MCD in BPH (ρ = 0.54 p = 0.04). Our data support the importance of c-Kit in regulating MC proliferation. The predominant location of MCs in peritumoral areas of canine PC was similar to the human counterpart, in which PC cells are supposed to produce substances attracting MCs to the tumor microenvironment. View Full-Text
Keywords: tumor microenvironment; mast cells; prostate cancer; dog tumor microenvironment; mast cells; prostate cancer; dog
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Defourny, S.V.P.; Romanucci, M.; Grieco, V.; Quaglione, G.R.; Santolini, C.; Della Salda, L. Tumor–Microenvironment Interaction: Analysis of Mast Cell Populations in Normal Tissue and Proliferative Disorders of the Canine Prostate. Vet. Sci. 2019, 6, 16.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Vet. Sci. EISSN 2306-7381 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top