Comparative Study of a Cell-Based and Electrochemical Biosensor for the Rapid Detection of 2,4,6-Trichloroanisole in Barrel Water Extracts
Abstract
:1. Introduction
- To be able to detect the presence of 2,4,6-TCA below or above the cut-off concentration of 2.5 ng/L.
- To be operable in a rapid and cost-effective manner.
- To become the basis, within the framework of further development, of a high throughput device that can be used in line with the production chain.
2. Materials and Methods
2.1. Reagents
2.2. Sample Source, Composition, and Processing
2.3. Biosensor Device
2.4. Assay Setup
2.4.1. Potentiometry Approach
Cell Culture and Manufacturing of Biorecognition Elements
Spiking Haloanisole-Free Water Extract Samples
Assay Procedure
2.4.2. Cyclic Voltammetry Approach
Sample Preparation
Electrochemical Measurements
Calibration Plot and the Interpretation of Results
2.5. Statistical Design
3. Results
3.1. Biosensor Response to the Presence of 2,4,6-TCA in Oak Barrel Water Extracts
3.2. Results of Potentiometric Biosensor Testing with Other Versions of the Cell Biorecognition Elements
3.3. Cyclic Voltammetry Analysis
4. Discussion
5. Conclusions
- The validation of the method with actual samples in the framework of a double-blind trial
- The investigation of the extent of possible interference by compounds structurally related to 2,4,6-TCA (e.g., other haloanisoles)
- The establishment of the range of quantitative determination
Author Contributions
Funding
Conflicts of Interest
References
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Moschopoulou, G.; Mavrikou, S.; Valdes, D.; Kintzios, S. Comparative Study of a Cell-Based and Electrochemical Biosensor for the Rapid Detection of 2,4,6-Trichloroanisole in Barrel Water Extracts. Beverages 2019, 5, 1. https://doi.org/10.3390/beverages5010001
Moschopoulou G, Mavrikou S, Valdes D, Kintzios S. Comparative Study of a Cell-Based and Electrochemical Biosensor for the Rapid Detection of 2,4,6-Trichloroanisole in Barrel Water Extracts. Beverages. 2019; 5(1):1. https://doi.org/10.3390/beverages5010001
Chicago/Turabian StyleMoschopoulou, Georgia, Sophie Mavrikou, Diego Valdes, and Spyridon Kintzios. 2019. "Comparative Study of a Cell-Based and Electrochemical Biosensor for the Rapid Detection of 2,4,6-Trichloroanisole in Barrel Water Extracts" Beverages 5, no. 1: 1. https://doi.org/10.3390/beverages5010001