In Vitro Antioxidant Activity of Purple Grape Juice (Vitis Labrusca) against Temozolamide Treatment in Rat Brains
Abstract
:1. Introduction
2. Materials and Methods
2.1. Compounds and Reagents
2.2. Grape Juice Chemical Evaluation and Phenolic Compound Content
2.3. Animals
2.4. Tissue Preparation
2.5. Incubation
2.6. Parameters of Oxidative Stress
2.7. Protein Determination
2.8. Statistical Analysis
3. Results
3.1. Grape Juice Composition
3.2. Effect of TMZ in Pre-Treatment and Co-Treatment with Purple Grape Juice on Oxidative Stress Parameters in Different Brain Structures of Rats
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameter | Purple Grape Juice |
---|---|
Total acidity (mEq/L) | 109.00 ± 0.01 |
Total volatile acidity (mEq/L) | 3.00 ± 0.01 |
Total carbohydrates (g/L) | 141.24 ± 0.01 |
Density (20 °C) | 1.062 ± 0.01 |
pH | 3.53 ± 0.08 |
Phenolic Compound (mg/L) | Purple Grape Juice |
---|---|
Total phenolic compound | 17.88 ± 0.28 |
Catechin | 1.17 ± 0.01 |
Epicatechin | 5.02 ± 0.06 |
Resveratrol | 0.24 ± 0.01 |
Naringin | 38.23 ± 0.48 |
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Gabardo, T.; Frusciante, M.R.; Pereira Marinho, J.; Dos Santos, M.; Abujamra, A.L.; Dani, C.; Funchal, C. In Vitro Antioxidant Activity of Purple Grape Juice (Vitis Labrusca) against Temozolamide Treatment in Rat Brains. Beverages 2018, 4, 99. https://doi.org/10.3390/beverages4040099
Gabardo T, Frusciante MR, Pereira Marinho J, Dos Santos M, Abujamra AL, Dani C, Funchal C. In Vitro Antioxidant Activity of Purple Grape Juice (Vitis Labrusca) against Temozolamide Treatment in Rat Brains. Beverages. 2018; 4(4):99. https://doi.org/10.3390/beverages4040099
Chicago/Turabian StyleGabardo, Tatiane, Marina Rocha Frusciante, Jessica Pereira Marinho, Manuela Dos Santos, Ana Lúcia Abujamra, Caroline Dani, and Cláudia Funchal. 2018. "In Vitro Antioxidant Activity of Purple Grape Juice (Vitis Labrusca) against Temozolamide Treatment in Rat Brains" Beverages 4, no. 4: 99. https://doi.org/10.3390/beverages4040099