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Article

Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical Screening

1
Aquatic and Crop Resource Development, National Research Council of Canada, Halifax, NS B3H 3Z1, Canada
2
New Substances Assessment Control Bureau, Health Canada, Ottawa, ON K1A 0K9, Canada
*
Author to whom correspondence should be addressed.
Toxics 2020, 8(4), 126; https://doi.org/10.3390/toxics8040126
Received: 30 November 2020 / Revised: 10 December 2020 / Accepted: 11 December 2020 / Published: 21 December 2020
(This article belongs to the Special Issue Advanced Zebrafish Model for Environmental Health Sciences Research)
The movement away from mammalian testing of potential toxicants and new chemical entities has primarily led to cell line testing and protein-based assays. However, these assays may not yet be sufficient to properly characterize the toxic potential of a chemical. The zebrafish embryo model is widely recognized as a potential new approach method for chemical testing that may provide a bridge between cell and protein-based assays and mammalian testing. The Zebrafish Embryo Toxicity (ZET) model is increasingly recognized as a valuable toxicity testing platform. The ZET assay focuses on the early stages of embryo development and is considered a more humane model compared to adult zebrafish testing. A complementary model has been developed that exposes larvae to toxicants at a later time point during development where body patterning has already been established. Here we compare the toxicity profiles of 20 compounds for this General and Behavioral Toxicity (GBT) assay to the ZET assay. The results show partially overlapping toxicity profiles along with unique information provided by each assay. It appears from this work that these two assays applied together can strengthen the use of zebrafish embryos/larvae as standard toxicity testing models. View Full-Text
Keywords: zebrafish larvae; toxicity assay; phenotype; developmental period zebrafish larvae; toxicity assay; phenotype; developmental period
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MDPI and ACS Style

Achenbach, J.C.; Leggiadro, C.; Sperker, S.A.; Woodland, C.; Ellis, L.D. Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical Screening. Toxics 2020, 8, 126. https://doi.org/10.3390/toxics8040126

AMA Style

Achenbach JC, Leggiadro C, Sperker SA, Woodland C, Ellis LD. Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical Screening. Toxics. 2020; 8(4):126. https://doi.org/10.3390/toxics8040126

Chicago/Turabian Style

Achenbach, John C., Cindy Leggiadro, Sandra A. Sperker, Cindy Woodland, and Lee D. Ellis. 2020. "Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical Screening" Toxics 8, no. 4: 126. https://doi.org/10.3390/toxics8040126

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