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Article

A Real-Time PCR Method for the Authentication of Common Cuttlefish (Sepia officinalis) in Food Products

Instituto de Investigaciones Marinas (IIM-CSIC), Eduardo Cabello 6, 36208 Vigo (Pontevedra), Spain
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Foods 2020, 9(3), 286; https://doi.org/10.3390/foods9030286
Received: 6 February 2020 / Revised: 27 February 2020 / Accepted: 28 February 2020 / Published: 4 March 2020
Cephalopods are very relevant food resources. The common cuttlefish (Sepia officinalis) is highly appreciated by consumers and there is a lack of rapid methods for its authentication in food products. We introduce a new minor groove binding (MGB) TaqMan real-time PCR (Polymerase Chain Reaction) method for the authentication of S. officinalis in food products to amplify a 122 base pairs (bp) fragment of the mitochondrial COI (Cytochrome Oxidase I) region. Reference and commercial samples of S. officinalis showed a threshold cycle (Ct) mean of 14.40, while the rest of the species examined did not amplify, or showed a significantly different Ct (p < 0.001). The calculated efficiency of the system was 101%, and the minimum DNA quantity detected was 10−4 ng. No cross-reactivity was detected with any other species, thus, the designed method differentiates S. officinalis from other species of the genus Sepia and other cephalopod species and works for fresh, frozen, grilled, cooked and canned samples of Sepia spp. The method has proved to be reliable and rapid, and it may prove to be a useful tool for the control of fraud in cuttlefish products. View Full-Text
Keywords: Sepia; common cuttlefish; Sepia officinalis; real-time PCR (Polymerase Chain Reaction); species identification; food authentication; COI (Cytochrome Oxidase I) Sepia; common cuttlefish; Sepia officinalis; real-time PCR (Polymerase Chain Reaction); species identification; food authentication; COI (Cytochrome Oxidase I)
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MDPI and ACS Style

Velasco, A.; Ramilo-Fernández, G.; Sotelo, C.G. A Real-Time PCR Method for the Authentication of Common Cuttlefish (Sepia officinalis) in Food Products. Foods 2020, 9, 286. https://doi.org/10.3390/foods9030286

AMA Style

Velasco A, Ramilo-Fernández G, Sotelo CG. A Real-Time PCR Method for the Authentication of Common Cuttlefish (Sepia officinalis) in Food Products. Foods. 2020; 9(3):286. https://doi.org/10.3390/foods9030286

Chicago/Turabian Style

Velasco, Amaya, Graciela Ramilo-Fernández, and Carmen G. Sotelo 2020. "A Real-Time PCR Method for the Authentication of Common Cuttlefish (Sepia officinalis) in Food Products" Foods 9, no. 3: 286. https://doi.org/10.3390/foods9030286

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