Next Article in Journal
Consumer Description by Check-All-That-Apply Questions (CATA) of the Sensory Profiles of Commercial and New Mandarins. Identification of Preference Patterns and Drivers of Liking
Next Article in Special Issue
Flash Gas Chromatography in Tandem with Chemometrics: A Rapid Screening Tool for Quality Grades of Virgin Olive Oils
Previous Article in Journal
Changes in Polyphenolics during Storage of Products Prepared with Freeze-Dried Wild Blueberry Powder
Previous Article in Special Issue
A Real-Time PCR Method for the Authentication of Common Cuttlefish (Sepia officinalis) in Food Products
Article

Genetic Identification of the Wild Form of Olive (Olea europaea var. sylvestris) Using Allele-Specific Real-Time PCR

Department of Chemistry, University of Patras, 26504 Rio Patras, Greece
*
Author to whom correspondence should be addressed.
Foods 2020, 9(4), 467; https://doi.org/10.3390/foods9040467
Received: 28 February 2020 / Revised: 4 April 2020 / Accepted: 7 April 2020 / Published: 9 April 2020
The wild-type of olive tree, Olea europaea var Sylvestris or oleaster, is the ancestor of the cultivated olive tree. Wild-type olive oil is considered to be more nutritious with increased antioxidant activity compared to the common cultivated type (Olea europaea L. var Europaea). This has led to the wild-type of olive oil having a much higher financial value. Thus, wild olive oil is one of the most susceptible agricultural food products to adulteration with other olive oils of lower nutritional and economical value. As cultivated and wild-type olives have similar phenotypes, there is a need to establish analytical methods to distinguish the two plant species. In this work, a new method has been developed which is able to distinguish Olea europaea var Sylvestris (wild-type olive) from Olea europaea L. var Europaea (cultivated olive). The method is based, for the first time, on the genotyping, by allele-specific, real-time PCR, of a single nucleotide polymorphism (SNP) present in the two olives’ chloroplastic genomes. With the proposed method, we were able to detect as little as 1% content of the wild-type olive in binary DNA mixtures of the two olive species. View Full-Text
Keywords: Olea europaea var Sylvestris; oleaster; olive; olive oil; real-time PCR; adulteration; SNP; DNA Olea europaea var Sylvestris; oleaster; olive; olive oil; real-time PCR; adulteration; SNP; DNA
Show Figures

Graphical abstract

MDPI and ACS Style

Kyriakopoulou, C.I.; Kalogianni, D.P. Genetic Identification of the Wild Form of Olive (Olea europaea var. sylvestris) Using Allele-Specific Real-Time PCR. Foods 2020, 9, 467. https://doi.org/10.3390/foods9040467

AMA Style

Kyriakopoulou CI, Kalogianni DP. Genetic Identification of the Wild Form of Olive (Olea europaea var. sylvestris) Using Allele-Specific Real-Time PCR. Foods. 2020; 9(4):467. https://doi.org/10.3390/foods9040467

Chicago/Turabian Style

Kyriakopoulou, Christina I., and Despina P. Kalogianni 2020. "Genetic Identification of the Wild Form of Olive (Olea europaea var. sylvestris) Using Allele-Specific Real-Time PCR" Foods 9, no. 4: 467. https://doi.org/10.3390/foods9040467

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop