Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn Tea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Extraction of Crude Polysaccharides
2.3. Purification of Crude Polysaccharide
2.4. Ultraviolet and FT-IR Analysis
2.5. Molecular Weight Analysis
2.6. Monosaccharide Analysis
2.7. Methylation Analysis
2.8. Nuclear Magnetic Resonance (NMR) Analysis
2.9. Scanning Electronic Microscopy (SEM) Analysis
2.10. Antioxidant Experiments
2.10.1. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) Scavenging Experiment
2.10.2. •OH Scavenging Experiment
2.10.3. ABTS+ Scavenging Experiment
2.11. Statistical Analysis
3. Results and Discussion
3.1. Extraction and Purification of Polysaccharide from Summer–Autumn Tea
3.2. UV and FT-IR Analysis of D1N1
3.3. Molecular Weight (Mw) of D1N1
3.4. Monosaccharide Analysis of D1N1
3.5. Linkage Analysis of D1N1
3.6. NMR Analysis of D1N1
3.7. SEM Analysis of D1N1
3.8. Antioxidant Activity of D1N1 In Vitro
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | DIN1 | |||
---|---|---|---|---|
Monosaccharide composition | Rhamnose | Arabinose | Galactose | Glucose |
Molar ratio (%) | 0.44 | 2.43 | 1.83 | 95.30 |
Sample | Type of Linkage | Methylated Sugars | Retention Time | Mass Fragments | Relative Molar Ratio (%) |
---|---|---|---|---|---|
D1N1 | t-Glc(p) | 1,5-di-O-acetyl-2,3,4,6-tetra-O-methyl glucitol | 8.994 | 55.1, 71.1, 87.1, 102.1, 129.1, 145.1, 162.1, 205.1, 239.3 | 11.83 |
4-Glc(p) | 1,4,5-tri-O-acetyl-2,3,6-tri-O-methyl glucitol | 14.244 | 71, 87, 102, 118, 129, 142, 162, 233 | 82.36 | |
3,4-Gal(p) | 1,3,4,5-tetra-O-acetyl-2,6-di-O-methyl galactitol | 16.308 | 59, 87, 99, 118, 129, 143, 185 | 2.31 | |
4,6-Glc(p) | 1,4,5,6-tetra-O-acetyl-2,3-tri-O-methyl glucitol | 18.414 | 59, 74, 85, 102, 118, 127, 142, 162, 201, 261 | 3.50 |
Code | Glycosyl Residues | Chemical Shifts (ppm) | |||||
---|---|---|---|---|---|---|---|
H1/C1 | H2/C2 | H3/C3 | H4/C4 | H5/C5 | H6/C6 | ||
A | →4)-α-d-Glcp-(1→ | 5.34 | 3.57 | 3.98 | 3.60 | 3.77 | 3.62, 3.78 |
99.61 | 71.53 | 70.4 | 76.81 | 71.18 | 60.45 | ||
B | α-d-Glcp-(1→ | 4.91 | 3.53 | 3.90 | 3.36 | 3.57 | 3.62, 3.92 |
98.61 | 71.40 | 73.31 | 69.31 | 71.53 | 60.79 | ||
C | →3,4)-α-d-Galp-(1→ | 5.16 | 3.50 | 3.76 | 3.90 | 3.49 | 3.65, 3.90 |
91.89 | 70.00 | 76.78 | 76.64 | 70.30 | 61.30 | ||
D | →4,6)-α- d -Glcp-(1→ | 5.28 | 3.58 | 3.87 | 3.53 | 3.90 | 3.87 |
99.73 | 72.52 | 71.33 | 76.81 | 71.82 | 69.80 |
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Cao, M.; Cao, Z.; Tian, J.; Lv, W.; Wang, H. Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn Tea. Foods 2024, 13, 821. https://doi.org/10.3390/foods13060821
Cao M, Cao Z, Tian J, Lv W, Wang H. Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn Tea. Foods. 2024; 13(6):821. https://doi.org/10.3390/foods13060821
Chicago/Turabian StyleCao, Miao, Zheng Cao, Juanjuan Tian, Wenping Lv, and Hongxin Wang. 2024. "Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn Tea" Foods 13, no. 6: 821. https://doi.org/10.3390/foods13060821
APA StyleCao, M., Cao, Z., Tian, J., Lv, W., & Wang, H. (2024). Structural Characterization and In Vitro Antioxidant Activity of a Novel Polysaccharide from Summer–Autumn Tea. Foods, 13(6), 821. https://doi.org/10.3390/foods13060821