Microbiological Changes during Long-Storage of Beef Meat under Different Temperature and Vacuum-Packaging Conditions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Carcass Sampling and Experimental Treatments
2.2. Microbiological Determinations and DNA Extraction
2.3. 16S rRNA Amplicon Sequencing and Microbiome Data Analysis
2.4. Statistical Analysis of Microbiome Results
3. Results
3.1. Bacterial Growth in Vacuum-Packaged Chilled and Frozen Beef
3.2. Microbiome Richness and Evenness
3.3. Microbiome Composition
3.4. Prediction of Metabolic Functions of Microbiomes at the End of Storage
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristics | Number of Steers |
---|---|
Dentition (number of teeth) | |
2 | 26 |
4 | 14 |
Conformation a | |
A | 40 |
Degree of finishing b | |
1 | 12 |
2 | 28 |
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Rovira, P.; Brugnini, G.; Rodriguez, J.; Cabrera, M.C.; Saadoun, A.; de Souza, G.; Luzardo, S.; Rufo, C. Microbiological Changes during Long-Storage of Beef Meat under Different Temperature and Vacuum-Packaging Conditions. Foods 2023, 12, 694. https://doi.org/10.3390/foods12040694
Rovira P, Brugnini G, Rodriguez J, Cabrera MC, Saadoun A, de Souza G, Luzardo S, Rufo C. Microbiological Changes during Long-Storage of Beef Meat under Different Temperature and Vacuum-Packaging Conditions. Foods. 2023; 12(4):694. https://doi.org/10.3390/foods12040694
Chicago/Turabian StyleRovira, Pablo, Giannina Brugnini, Jesica Rodriguez, María C. Cabrera, Ali Saadoun, Guillermo de Souza, Santiago Luzardo, and Caterina Rufo. 2023. "Microbiological Changes during Long-Storage of Beef Meat under Different Temperature and Vacuum-Packaging Conditions" Foods 12, no. 4: 694. https://doi.org/10.3390/foods12040694