Comparison of Real-Time PCR and Droplet Digital PCR for the Quantitative Detection of Lactiplantibacillus plantarum subsp. plantarum

Choi, Chang-Hun; Kim, Eiseul; Yang, Seung-Min; Kim, Da-Som; Suh, Seung-Man; Lee, Ga-Young; Kim, Hae-Yeong

doi:10.3390/foods11091331

Open AccessArticle

Comparison of Real-Time PCR and Droplet Digital PCR for the Quantitative Detection of Lactiplantibacillus plantarum subsp. plantarum

by

Chang-Hun Choi

^†

,

Eiseul Kim

^†

,

Seung-Min Yang

,

Da-Som Kim

,

Seung-Man Suh

,

Ga-Young Lee

and

Hae-Yeong Kim

^*

Resources and Department of Food Science and Biotechnology, Institute of Life Sciences, Kyung Hee University, Yongin 17104, Korea

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to the work.

Foods 2022, 11(9), 1331; https://doi.org/10.3390/foods11091331

Submission received: 21 March 2022 / Revised: 26 April 2022 / Accepted: 30 April 2022 / Published: 3 May 2022

(This article belongs to the Special Issue PCR in Food Science: Current Technology and Applications)

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Abstract

:

Droplet digital polymerase chain reaction (ddPCR) is one of the newest and most promising tools providing absolute quantification of target DNA molecules. Despite its emerging applications in microorganisms, few studies reported its use for detecting lactic acid bacteria. This study evaluated the applicability of a ddPCR assay targeting molecular genes obtained from in silico analysis for detecting Lactiplantibacillus plantarum subsp. plantarum, a bacterium mainly used as a starter or responsible for fermentation in food. The performance characteristics of a ddPCR were compared to those of a quantitative real-time PCR (qPCR). To compare the linearity and sensitivity of a qPCR and ddPCR, the calibration curve for a qPCR and the regression curve for a ddPCR were obtained using genomic DNA [10²–10⁸ colony-forming units (CFU)/mL] extracted from a pure culture and spiked food sample. Both the qPCR and ddPCR assays exhibited good linearity with a high coefficient of determination in the pure culture and spiked food sample (R² ≥ 0.996). The ddPCR showed a 10-fold lower limit of detection, suggesting that a ddPCR is more sensitive than a qPCR. However, a ddPCR has limitations in the absolute quantitation of high bacterial concentrations (>10⁶ CFU/mL). In conclusion, a ddPCR can be a reliable method for detecting and quantifying lactic acid bacteria in food.

Keywords:

Lactiplantibacillus plantarum; real-time PCR; droplet digital PCR; detection; quantification; fermented food

Graphical Abstract

1. Introduction

Lactic acid bacteria are involved in the spontaneous fermentations of foods such as meat, milk, fish, and vegetables [1]. They are commensal inhabitants of the human gastrointestinal tract and contribute to human health. As probiotics, lactic acid bacteria have shown that they may present beneficial effects, such as preventing diarrhea and inflammatory bowel disease [1,2,3]. The quantification of lactic acid bacteria is important for epidemiologic studies and their roles in various niche markets.

Lactiplantibacillus plantarum is a versatile species encountered in various niches, including meat, dairy, fish products, vegetables, and the human gastrointestinal tract [4,5]. L. plantarum is considered a safe probiotic, so it is widely used as an ingredient in fermented food and feed, such as cheese, milk, sauerkraut, and kimchi [6,7]. Between the two Lactiplantibacillus plantarum subspecies, L. plantarum subsp. plantarum provides a beneficial effect for the immune system, such as treating inflammatory diseases and mitigating pathogenic infections [8,9]. In a previous study, L. plantarum subsp. plantarum and Lactiplantibacillus plantarum subsp. argentoratensis affected the fermentation stage of vegetables, such as kimchi. L. plantarum subsp. plantarum was isolated only in fermented kimchi at a low temperature (4 °C), whereas L. plantarum subsp. argentoratensis was found only at a relatively high temperature (15 °C or 25 °C) [10]. Unlike L. plantarum subsp. plantarum, L. plantarum subsp. argentoratensis could not metabolize either methyl α-d-mannoside or melezitose [11].

A rapid method for the detection and quantification of L. plantarum subsp. plantarum in the food matrix is an essential tool for the food industry. However, prokaryotic systematics currently rely on labor- and time-consuming taxonomic approaches, including phenotypic characterization, variation analysis of 16S rRNA sequences, and DNA–DNA hybridization. Distinguishing closely related subspecies using these tools is difficult and often results in the misidentification of microorganisms [10]. Moreover, the molecular methods available for monitoring species or subspecies of L. plantarum are insufficient, as previous methods detect nontarget species of Lactiplantibacillus species or subspecies, including L. plantarum subsp. argentoratensis, Lactiplantibacillus paraplantarum, and Lactiplantibacillus pentosus [12,13]. Due to the limitations of these previous studies, there is an increasing demand for improving the current methods in studying prokaryotic systems [10].

The molecular-based detection and quantification of microorganisms have been successfully explored by real-time quantitative polymerase chain reaction (qPCR) in various food matrices [12,14]. A major advantage of a qPCR is that an amplification curve can be confirmed in hours instead of days, unlike conventional detection methods [15]. In addition, a qPCR is most commonly used as an efficient tool due to its high specificity and sensitivity [16]. A qPCR is a reliable and sensitive molecular tool applied and adopted in many different fields for the detection and quantification of genetically modified organisms (GMOs) [17,18], as well as for mutations and single nucleotide polymorphisms (SNPs) genotyping in the control of animal disease [19,20]. Recently, novel PCR-based methods for detecting and quantifying the molecular target have been introduced [21]. The droplet digital PCR (ddPCR) is a third-generation PCR tool [15]. A ddPCR mixture is divided into several partitions, each containing zero or at least one copy of the genomic DNA [15]. After amplification, partitions are counted as positive (presence of target gene) or negative (absence of target gene). The absolute quantification of the number of copies is performed using binomial Poisson statistics [15,22]. This allows a ddPCR to perform absolute quantification without using the calibration curve [15]. A ddPCR has been applied previously in various fields to quantify and detect genomic DNA targets, such as GMOs, viruses, pathogenic bacteria, antibiotic resistance genes, and vertebrate [23,24,25,26,27,28,29].

This study applied a ddPCR assay to detect and quantify L. plantarum subsp. plantarum in a food sample and compared the specificity and sensitivity of a qPCR targeting the ydiC gene. Moreover, the applicability of a ddPCR to detect lactic acid bacteria was discussed.

2. Materials and Methods

2.1. Bacterial Strains

L. plantarum subsp. plantarum and nontarget reference strains applied in this study were obtained from the Korean Collection for Type Cultures (KCTC, Daejeon, Korea), the Korean Culture Center of Microorganisms (KCCM, Seoul, Korea), the Korean Agricultural Culture Collection (KACC, Jeonju, Korea), the NITE Biological Resource Center (NBRC, Chiba, Japan), the Microorganism and Gene Bank (MGB, Gwangju, Korea), and the National Culture Collection for Pathogens (NCCP, Cheongju, Korea) (Table 1). All tested strains were grown in lactobacilli MRS (Difco Laboratories, Sparks, MD, USA) broth at 37 °C for 48 h. Genomic DNA of reference strains was extracted using DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany). The genomic DNA concentration and quality were measured using a Maestrogen Nano spectrophotometer (Maestrogen, Las Vegas, NV, USA). Genomic DNA was stored at −20 °C before use as a template for qPCR and ddPCR [30].

2.2. Primer and Probe Design

The ydiC gene (accession no. EFK30629.1) discovered by a pangenome analysis [12] was used as a target for detecting L. plantarum subsp. plantarum. The primer/probe set for detecting the ydiC gene was designed using the Primer Designer program (Scientific and Education Software, Durham, NC, USA). The primer and probe were designed considering the guanine-cytosine (GC) content and length to ensure high amplification conditions. In silico specificity was performed using in silico PCR amplification software (http://insilico.ehu.es/PCR/ accessed on 8 March 2022) [31] with genome sequences obtained from the GenBank sequence database. The sequences of the primer and probe used for qPCR and ddPCR assays and the amplicon size of the target gene are listed in Table 2.

2.3. qPCR Assay

The qPCR mixture consisted of 20 ng DNA template, 500 nM of each primer, 250 nM probe, 10 µL TaqMan™ Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA), and purified water to a final volume of 20 µL. The amplification reaction was performed with the 7500 Fast Real-time PCR System (Applied Biosystems). The reaction was run at 50, hold for 2 min, followed by 95 °C for 10 min, then 40 cycles consisting of 95 °C for 15 s and 60 °C for 1 min per cycle. The output data were analyzed using ABI 7500 Fast Software (Applied Biosystems, Foster City, CA, USA). The specificity of primer and probe set was confirmed using 105 reference strains (Table 1). A calibration curve was constructed using genomic DNA from L. plantarum subsp. plantarum KACC 11451 with different concentrations [10²–10⁸ colony-forming units (CFU)/mL], as reported previously [32]. The viable cell was counted by the plate count method. Briefly, serial dilutions of cultured strain were grown on MRS agar and counted after incubation at 37 °C for 48 h. Amplification was performed thrice to construct the calibration curve. The calibration curves were constructed by plotting the Ct value and the number of cells.

2.4. ddPCR Assay

ddPCR was performed using 10 µL of 2 × ddPCR Supermix for probe (Bio-Rad, Pleasanton, CA, USA), 500 nM of each primer, 250 nM probe, 20 ng DNA template, and distilled water to a total volume of 20 µL. ddPCR was used to make the droplet mixture using the QX200 droplet generator (Bio-Rad). The droplet mixture was transferred to a PCR reaction plate and amplified with the following conditions: denaturation of 95 °C for 10 min, followed by 40 cycles of a two-step thermal profile consisting of 95 °C for 15 s and 60 °C for 60 s. The PCR product was incubated at 98 °C for 10 min and cooled to 4 °C until the droplets were read. The PCR reaction plate was transferred to the QX200 droplet reader (Bio-Rad). The number of positive (high level of fluorescence) and negative (low and constant level of fluorescence) droplets obtained were analyzed using QuantaSoft software (Bio-Rad, Pleasanton, CA, USA) [33].

2.5. Artificially Contaminated Milk Sample

To compare the performance characteristics of qPCR and ddPCR, L. plantarum subsp. plantarum was used to artificially contaminate food samples. The food sample was obtained from a market and confirmed absent of L. plantarum subsp. plantarum by qPCR. To prepare the spiked food sample, the milk sample was spiked with a pure culture of L. plantarum subsp. plantarum at a concentration of 10⁸ CFU/mL [34,35] and mixed for 2 min using a homogenizer (Stomacher Circulator 400; Seward Ltd., London, UK). The number of bacteria was determined by the plate counting method according to a previous study [32]. Briefly, 0.1 mL of an appropriate dilution of bacteria was spread on MRS agar and incubation at 37 °C for 48 h for bacterial cell counting. An aliquot of 1 mL spiked food sample was transferred to a sterilized tube, used for genomic DNA extraction, and subjected to qPCR and ddPCR assays.

3. Results and Discussion

3.1. Specificity of Primer by In Silico PCR

Before performing a qPCR and ddPCR, the inclusivity and exclusivity of the new primer and probe set designed from the ydiC gene were tested by an in silico PCR assay. The ydiC gene is a novel genetic marker for detecting L. plantarum subsp. plantarum obtained from a pangenome analysis [12]. The sequence identity with the primer, in silico PCR result, and amplicon size obtained from the ydiC gene of 56 L. plantarum subsp. plantarum and 140 other species are represented in Table S1. The primer and probe generated a positive reaction with all L. plantarum subsp. plantarum strains, whereas the remaining nontarget species or subspecies produced a negative reaction. The amplicon size of all L. plantarum subsp. plantarum was 150 bp.

3.2. Evaluation of the Specificity and Sensitivity by qPCR

The accuracy of a qPCR and ddPCR depends on the specificity of the sequence or primer used in the experiment [36]. Many studies have reported detecting L. plantarum using the 16S rRNA sequence or housekeeping genes (atpD, recA, and dnaK) as qPCR marker genes [36,37,38]. However, these genes reported to date have high sequence homologies among other species or subspecies, such as L. plantarum subsp. argentoratensis and L. paraplantarum, and require an additional procedure for identification that is time-consuming and costly. Therefore, this study designed the primer and probe using the ydiC gene discovered by a pangenome analysis [12].

A qPCR was performed using L. plantarum subsp. plantarum and 104 other reference strains to determine the amplification efficiency and specificity of the primer targeting the ydiC gene. The target DNA of L. plantarum subsp. plantarum was successfully amplified (Figure 1A), whereas no amplification was observed for the 104 other reference strains tested, indicating that the primer was specific for L. plantarum subsp. plantarum detection, consistent with a previous result using the same target gene [12]. The calibration curve was constructed using the genomic DNA of L. plantarum subsp. plantarum ranging from 10⁸ to 10³ CFU/mL. When the calibration curves of a qPCR have R² ≥ 0.98 and a slope from −3.1 to −3.6, it can be regarded as a high-efficiency primer [39]. A calibration curve had an amplification efficiency of 88.364% (Figure 1B), suggesting that the primer showed a high efficiency in detecting L. plantarum subsp. plantarum.

Propidium monoazide (PMA) combined with a PCR appears to be a potential method for distinguishing between living and dead cells [40,41,42]. Several studies have reported the application of PMA treatments to quantify viable bacterial cells in foods by a qPCR and ddPCR [40,43]. In this study, because genomic DNA was quantified without a PMA treatment, there is a disadvantage that viable, but non-cultivable (VBNC) cells cannot be quantified.

3.3. Evaluation of Specificity and Sensitivity by ddPCR

A ddPCR has the potential to be a robust method for the quantification and detection of microorganisms in food [44,45]. This study investigated the potential of a ddPCR for detecting L. plantarum subsp. plantarum. Similar to the qPCR assay, the high specificity of a primer was observed in the ddPCR method. The genomic DNA from the 105 strains was detected by a ddPCR, and no droplets were observed for the 104 nontarget reference strains, including Lactiplantibacillus species closely related to L. plantarum subsp. plantarum (Figure 2A). Moreover, the ddPCR assay was sensitive with good linearity (slope = 0.9197, R² = 0.996 for detecting the ydiC gene) ranging from 10⁸ to 10² CFU/mL (Figure 2B). The limit of detection in the genomic DNA obtained from the pure culture was 10² CFU/mL, with a quantification value of 0.4 ± 0.11 copies (mean ± standard deviation). However, the ddPCR analysis failed to quantify the DNA when the L. plantarum subsp. plantarum population was >10⁶ CFU/mL (Table 3). In the ddPCR, reaction saturation was reached with more than 20,000 positive droplets, making it impossible to quantify this concentration [35].

3.4. Comparison of Sensitivity and Linearity of qPCR and ddPCR Assays

To compare the sensitivity and reliability, serial dilutions (10⁸–10² CFU/mL) of the genomic DNA extracted from the pure culture and spiked food sample were used to determine the limit of detection and the limit of quantification. The limit of detection and the limit of quantification were calculated according to the previous studies [39,46]. The limit of detection of the qPCR was determined as 10³ CFU/mL in both the pure culture and spiked food sample (Table 3). On the other hand, the limit of quantification was determined as 10⁴ CFU/mL (Table 3). The qPCR showed a good linearity range of 10⁸–10³ CFU/mL with a 0.999 coefficient of determination (R²) in the genomic DNA of the pure culture and spiked food sample (Figure 3A,B). In contrast to the qPCR, the quantitative detection range of the ddPCR was from 10⁶ to 10² CFU/mL (Table 3). The ddPCR assay showed the genomic DNA of the pure culture and spiked food sample with a good linearity (R² = 0.996 and 0.998; Figure 3C,D). The ddPCR assay exhibited the lowest limit of detection value (10² CFU/mL) and limit of quantification value (10³ CFU/mL) compared to the qPCR, showing that the ddPCR sensitivity was ten times higher than the qPCR detection (Table 3), consistent with previous studies in which the ddPCR is 10-fold more sensitive than the qPCR [15,44]. However, ddPCR droplets are positively saturated at >10⁶ CFU/mL bacterial concentrations, making the Poisson distribution estimation invalid and resulting in a narrower dynamic range than the qPCR. The limitation of the ddPCR reported in previous studies was the quantification of the target DNA when bacterial concentrations exceeded 10⁶ CFU/mL [15,22,44]. In this study, samples with a high bacterial abundance (>10⁶ CFU/mL) were not quantified. To accurately quantify these samples, they must be diluted and run again on a ddPCR, thus increasing the time and cost required for the analysis [15]. Therefore, it is necessary to confirm that the amount of the target DNA is within the measurement range before performing the experiment.

This study evaluated the applicability of a ddPCR to detect L. plantarum subsp. plantarum. Unlike a qPCR, a ddPCR does not require a calibration curve. A ddPCR allowed quantifying the absolute number of the target DNA added to the mixture by partitioning the PCR reagents and using the Poisson algorithm [21,47]. This may reduce the bias introduced in the qPCR assay, as the calculated bacterial concentrations had to be constructed for the calibration curve. Moreover, the higher sensitivity of a ddPCR than of a qPCR has been reported previously [15,22,44]. This would be an advantage in food samples having PCR inhibitors or containing low copies of target molecules [27,28]. The difference in sensitivity between the assays was attributed to the higher resistance of the ddPCR to inhibitors occurring in food matrices [25,35]. In a ddPCR, the target DNA is distributed over thousands of droplets that constitute separate reaction compartments, leading to a higher tolerance to inhibitors [35,48]. Although more expensive and time-consuming than a qPCR, a ddPCR is currently a more reliable tool for detecting microorganisms, viruses, and GMOs [24,40,43,49]. Previous studies have demonstrated that a ddPCR is a suitable analytical tool for detecting foodborne pathogens in the food matrix [44,45,50]. Similar to foodborne pathogens, a ddPCR is a useful tool for detecting low-level L. plantarum subsp. plantarum. Therefore, a ddPCR can be a suitable analytical tool for the quantitative detection of lactic acid bacteria, which is important in probiotic products, dairy products, or fermented food.

4. Conclusions

To the best of our knowledge, this is the first report that demonstrated the ddPCR assay to quantify L. plantarum subsp. plantarum. The ddPCR observed a lower limit of detection than the qPCR, which could be advantageous in foods with a low number of target species. The ddPCR represents an innovation in the molecular world and is very useful, sensitive, and reliable for overcoming different limits for L. plantarum subsp. plantarum quantification. At the same time, it is not an instrument that is accessible and easy to use in any laboratories and industries, both for costs and for the type of analysis. In conclusion, this study can be used as preliminary data for a future robust assay optimization and validation. This method, which enables the quantification of L. plantarum subsp. plantarum in the food samples, can be a useful tool in the food industry to evaluate the quality of fermented food products.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/foods11091331/s1, Table S1: In silico PCR for specificity of primer and probe.

Author Contributions

Conceptualization, E.K. and H.-Y.K.; methodology, E.K. and S.-M.Y.; investigation, C.-H.C., S.-M.Y. and D.-S.K.; validation, S.-M.S. and G.-Y.L.; writing—original draft preparation, C.-H.C. and E.K.; writing—review and editing, H.-Y.K.; visualization, E.K.; project administration, H.-Y.K.; funding acquisition, H.-Y.K. All authors have read and agreed to the published version of the manuscript.

Funding

This work was carried out with the support of the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01662001)” Rural Development Administration, Republic of Korea.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data is contained within the article or supplementary material.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Assessment of primer specificity and sensitivity using qPCR. (A) Amplification plot of qPCR; (B) amplification plot of serial dilution of genomic DNA of L. plantarum subsp. plantarum KACC 11451.

Figure 2. Assessment of primer specificity and sensitivity using ddPCR. The positive and negative droplets classified as the thresholds of individual wells are indicated in blue and black, respectively. The threshold is determined by the droplet reader and is shown as a horizontal line. (A) Specificity of primer by ddPCR; (B) quantification of genomic DNA of L. plantarum subsp. plantarum KACC 11451 by ddPCR.

Figure 3. Linearity and sensitivity of two assays. (A) qPCR in analyzing genomic DNA of L. plantarum subsp. plantarum KACC 11451; (B) qPCR in analyzing spiked milk sample; (C) ddPCR in analyzing genomic DNA of L. plantarum subsp. plantarum KACC 11451; (D) ddPCR in analyzing spiked milk sample.

Table 1. List of 105 reference strains.

Species	Strain No.
Lactiplantibacillus plantarum subsp. plantarum	KACC 11451
Lactiplantibacillus plantarum subsp. argentoratensis	KACC 12404
Lactiplantibacillus paraplantarum	KACC 12373
Lactiplantibacillus pentosus	KACC 12428
Apilactobacillus kunkeei	KACC 19371
Bifidobacterium animalis subsp. animalis	KCTC 3125
Bifidobacterium animalis subsp. lactis	KCTC 5854
Bifidobacterium bifidum	KCTC 3418
Bifidobacterium bifidum	KCTC 3440
Bifidobacterium breve	KACC 16639
Bifidobacterium breve	KCTC 3419
Bifidobacterium longum subsp. infantis	KCTC 3249
Bifidobacterium longum subsp. longum	KCCM 11953
Companilactobacillus crustorum	KACC 16344
Companilactobacillus farciminis	KACC 12423
Companilactobacillus heilongjiangensis	KACC 18741
Enterococcus avium	NCCP 10761
Enterococcus avium	KACC 10788
Enterococcus casseliflavus	KCTC 3638
Enterococcus cecorum	KACC 13884
Enterococcus durans	KCTC 13289
Enterococcus durans	KACC 10787
Enterococcus faecalis	KCTC 3206
Enterococcus faecalis	KACC 11859
Enterococcus faecium	KCTC 13225
Enterococcus faecium	KACC 11954
Enterococcus faecium	KACC 10782
Enterococcus gilvus	KACC 13847
Enterococcus malodoratus	KACC 13883
Enterococcus mundtii	KCTC 3630
Enterococcus mundtii	KACC 13824
Enterococcus saccharolyticus	KACC 10783
Enterococcus thailandicus	KCTC 13134
Fructilactobacillus lindneri	KACC 12445
Lacticaseibacillus brantae	KACC 17260
Lacticaseibacillus camelliae	KACC 17261
Lacticaseibacillus casei	KACC12413
Lacticaseibacillus casei	KCTC 13086
Lacticaseibacillus casei	KCTC 3110
Lacticaseibacillus chiayiensis	NBRC 112906
Lacticaseibacillus manihotivorans	KACC 12380
Lacticaseibacillus pantheris	KACC 12395
Lacticaseibacillus paracasei subsp. paracasei	KCTC 3165
Lacticaseibacillus paracasei subsp. tolerans	KCTC 3074
Lacticaseibacillus rhamnosus	KACC 11953
Lacticaseibacillus rhamnosus	KCTC 13088
Lacticaseibacillus sharpeae	KACC 11462
Lactobacillus acetotolerans	KACC 12447
Lactobacillus acidophilus	KACC 12419
Lactobacillus acidophilus	KCTC 3164
Lactobacillus amylolyticus	KACC 12374
Lactobacillus amylophilus	KACC 11430
Lactobacillus amylovorus	KACC 12435
Lactobacillus brevis	KCTC 3498
Lactobacillus curvatus subsp. curvatus	KACC 12415
Lactobacillus delbrueckii subsp. bulgaricus	KACC 12420
Lactobacillus delbrueckii subsp. delbrueckii	KACC 13439
Lactobacillus delbrueckii subsp. lactis	KACC 12417
Lactobacillus gallinarum	KACC 12370
Lactobacillus gasseri	KCTC 3163
Lactobacillus gasseri	KACC 12424
Lactobacillus helveticus	KACC 12418
Lactobacillus jensenii	KCTC 5194
Lactobacillus johnsonii	KCTC 3801
Lactococcus lactis subsp. lactis	KCTC 3769
Lactococcus lactis subsp. lactis	KCTC 2013
Latilactobacillus sakei subsp. sakei	KCTC 3603
Lentilactobacillus buchneri	KACC 12416
Lentilactobacillus parabuchneri	KACC 12363
Leuconostoc carnosum	KCTC 3525
Leuconostoc citreum	KCTC 3526
Leuconostoc fallax	KACC 12303
Leuconostoc gelidum	KACC 12256
Leuconostoc gelidum subsp. aenigmaticum	MGB 1000TE
Leuconostoc gelidum subsp. gasicomitatum	KACC 13854
Leuconostoc gelidum subsp. gelidum	KCTC 3527
Leuconostoc holzapfelii	KACC 17729
Leuconostoc lactis	KCTC 3528
Leuconostoc mesenteroides subsp. dextranicum	KACC 12315
Leuconostoc mesenteroides subsp. mesenteroides	KCTC 3505
Leuconostoc pseudomesenteroides	KACC 12304
Levilactobacillus zymae	KACC 16349
Ligilactobacillus acidipiscis	KACC 12394
Ligilactobacillus agilis	KACC 12433
Ligilactobacillus ruminis	KACC 12429
Ligilactobacillus salivarius	KCTC 3600
Limosilactobacillus fermentum	KACC 11441
Limosilactobacillus fermentum	KCTC 3112
Limosilactobacillus fermentum	KCTC 5049
Limosilactobacillus mucosae	KACC 12381
Limosilactobacillus reuteri	KCTC 3594
Loigolactobacillus coryniformis subsp. coryniformis	KACC 12411
Streptococcus salivarius subsp. thermophilus	KACC 11857
Weissella beninensis	KACC 18586
Weissella cibaria	KCTC 3746
Weissella confusa	KACC 11841
Weissella halotolerans	KACC 11843
Weissella hellenica	KACC 11842
Weissella kandleri	KACC 11844
Weissella koreensis	KACC 11853
Weissella minor	KCTC 3604
Weissella paramesenteroides	KACC 11847
Weissella soli	KACC 11848
Weissella thailandensis	KACC 11849
Weissella viridescens	KACC 11850

Table 2. Information for primer and probe for detecting L. plantarum subsp. plantarum.

Name	Sequence (5′-3′)	Size (bp)
Plantarum_F	GGT GGC TGG TTG AGT GAT CT	150 bp
Plantarum_R	GCC GAT ACC GTT GGA AAT TA
Plantarum_P	FAM-ACA GCT TGT TCT ACT AAC CGG CCT AGT CC-MGB

Table 3. Quantification of genomic DNA extracted from pure culture and spiked milk sample.

Conc. (CFU/mL)	Pure Culture ¹		Spiked Food Sample
	qPCR (Ct)	ddPCR (Copies)	qPCR (Ct)	ddPCR (Copies)
10⁸	16.37 ± 0.04 (9)	Saturated ²	16.13 ± 0.08 (9)	Saturated
10⁷	19.83 ± 0.03 (9)	Saturated	19.52 ± 0.05 (9)	Saturated
10⁶	23.37 ± 0.02 (9)	3287.78 ± 106.98 (9)	23.46 ± 0.07 (9)	4310 ± 295 (9)
10⁵	27.08 ± 0.15 (9)	318.44 ± 10.49 (9)	27.44 ± 0.12 (9)	427.22 ± 60.31 (9)
10⁴	30.67 ± 0.08 (9)	31.64 ± 1.9 (9)	31.25 ± 0.16 (9)	40.46 ± 6.58 (9)
10³	34.11 ± 0.31 (9)	3.42 ± 0.62 (9)	34.79 ± 0.3 (9)	3.98 ± 0.59 (9)
10²	ND ³	0.4 ± 0.11 (9)	ND	0.25 ± 0.14 (9)

¹ Mean ± standard deviation (number of positive replicates on nine replicates analyzed). ² DNA concentration at which the signal of the ddPCR assay was saturated. ³ ND, not detectable.

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Choi, C.-H.; Kim, E.; Yang, S.-M.; Kim, D.-S.; Suh, S.-M.; Lee, G.-Y.; Kim, H.-Y. Comparison of Real-Time PCR and Droplet Digital PCR for the Quantitative Detection of Lactiplantibacillus plantarum subsp. plantarum. Foods 2022, 11, 1331. https://doi.org/10.3390/foods11091331

AMA Style

Choi C-H, Kim E, Yang S-M, Kim D-S, Suh S-M, Lee G-Y, Kim H-Y. Comparison of Real-Time PCR and Droplet Digital PCR for the Quantitative Detection of Lactiplantibacillus plantarum subsp. plantarum. Foods. 2022; 11(9):1331. https://doi.org/10.3390/foods11091331

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Choi, Chang-Hun, Eiseul Kim, Seung-Min Yang, Da-Som Kim, Seung-Man Suh, Ga-Young Lee, and Hae-Yeong Kim. 2022. "Comparison of Real-Time PCR and Droplet Digital PCR for the Quantitative Detection of Lactiplantibacillus plantarum subsp. plantarum" Foods 11, no. 9: 1331. https://doi.org/10.3390/foods11091331

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Choi, C.-H., Kim, E., Yang, S.-M., Kim, D.-S., Suh, S.-M., Lee, G.-Y., & Kim, H.-Y. (2022). Comparison of Real-Time PCR and Droplet Digital PCR for the Quantitative Detection of Lactiplantibacillus plantarum subsp. plantarum. Foods, 11(9), 1331. https://doi.org/10.3390/foods11091331

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