Understanding the Effect of Ozone on Listeria monocytogenes and Resident Microbiota of Gorgonzola Cheese Surface: A Culturomic Approach
Abstract
:1. Introduction
2. Materials and Methods
2.1. Gorgonzola Rind Samples
2.2. Inoculum Preparation
2.3. Inoculation of Rind Samples and Ozone Treatments
2.4. Microbiological Analysis and Determination of pH/aw
2.5. MALDI-TOF MS Identification and Culturomic Analysis
2.6. Statistical Analyses and Graphing
3. Results
3.1. Behaviour of L. monocytogenes and Resident Populations during Storage
3.2. Changes in pH and aw during Storage
3.3. Culturomic Analysis and MALDI-TOF MS Identification
3.3.1. Relative Abundances of Microbial Genera
3.3.2. Percentage of Colonies Identified at Species Level
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Internal ID | NCBI ID a | Isolation Source | Year | Lineage | Serogroup | Sequence Type | Clonal Complex |
---|---|---|---|---|---|---|---|
G40 | CFSAN044840 | Gorgonzola cheese | 2005 | II | IIa | 325 | 31 |
G52 | CFSAN044807 | Gorgonzola cheese | 2004 | II | Iia | 325 | 31 |
G70 | CFSAN044814 | Gorgonzola cheese | 2004 | II | Iia | 325 | 31 |
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Panebianco, F.; Rubiola, S.; Buttieri, C.; Di Ciccio, P.A.; Chiesa, F.; Civera, T. Understanding the Effect of Ozone on Listeria monocytogenes and Resident Microbiota of Gorgonzola Cheese Surface: A Culturomic Approach. Foods 2022, 11, 2640. https://doi.org/10.3390/foods11172640
Panebianco F, Rubiola S, Buttieri C, Di Ciccio PA, Chiesa F, Civera T. Understanding the Effect of Ozone on Listeria monocytogenes and Resident Microbiota of Gorgonzola Cheese Surface: A Culturomic Approach. Foods. 2022; 11(17):2640. https://doi.org/10.3390/foods11172640
Chicago/Turabian StylePanebianco, Felice, Selene Rubiola, Chiara Buttieri, Pierluigi Aldo Di Ciccio, Francesco Chiesa, and Tiziana Civera. 2022. "Understanding the Effect of Ozone on Listeria monocytogenes and Resident Microbiota of Gorgonzola Cheese Surface: A Culturomic Approach" Foods 11, no. 17: 2640. https://doi.org/10.3390/foods11172640