Next Article in Journal
Trends in Food Preferences and Sustainable Behavior during the COVID-19 Lockdown: Evidence from Spanish Consumers
Previous Article in Journal
Shelf-Life of Half-Shell Mussel (Mytilus edulis) as Affected by Pullulan, Acidic Electrolyzed Water, and Stable Chlorine Dioxide Combined Ice-Glazing during Frozen Storage
 
 
Article

Optimization and Validation of a New Microbial Inhibition Test for the Detection of Antimicrobial Residues in Living Animals Intended for Human Consumption

1
Instituto Agroalimentario de Aragón-IA2, Universidad de Zaragoza-CITA, 50013 Zaragoza, Spain
2
Department of R&D, ZEULAB S.L., 50197 Zaragoza, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Susana Casal
Foods 2021, 10(8), 1897; https://doi.org/10.3390/foods10081897
Received: 2 June 2021 / Revised: 28 July 2021 / Accepted: 11 August 2021 / Published: 16 August 2021
(This article belongs to the Section Food Quality and Safety)
Even though antibiotics are necessary in livestock production, they can be harmful not only due to their toxicity, but also in view of their contribution to the emergence of antimicrobial resistance. Screening tests based on microbial growth inhibition appeared to be useful tools to prevent its entry into the food chain. They have nevertheless been traditionally carried out post mortem, leading to great economical loss and harm to the environment in case a positive sample is found. Hence, the objective was to evaluate the use of a screening test as an ante mortem alternative for the detection of antibiotic residues in meat: thus, Explorer®-Blood test was optimized and validated. After adapting the procedure for matrix preparation, the assay parameters were assessed from 344 antibiotic-free blood serum samples. Limits of Detection (LoDs) were defined by spiking blood serum with several of the most common antimicrobials used in veterinary practice. LoDs were similar to those obtained for meat and were at or below the maximum residue limits set by EU legislation for muscle. Analyses of in vivo injected samples, previously characterized by LC-MS/MS, demonstrated the method’s accuracy and proved that Explorer®-Blood can be considered a suitable alternative to conventional post mortem screening methods. View Full-Text
Keywords: antibiotics; sulfonamides; antimicrobials; blood; in vivo; biological test antibiotics; sulfonamides; antimicrobials; blood; in vivo; biological test
Show Figures

Graphical abstract

MDPI and ACS Style

Serrano, M.J.; Mata, L.; García-Gonzalo, D.; Antón, A.; Razquin, P.; Condón, S.; Pagán, R. Optimization and Validation of a New Microbial Inhibition Test for the Detection of Antimicrobial Residues in Living Animals Intended for Human Consumption. Foods 2021, 10, 1897. https://doi.org/10.3390/foods10081897

AMA Style

Serrano MJ, Mata L, García-Gonzalo D, Antón A, Razquin P, Condón S, Pagán R. Optimization and Validation of a New Microbial Inhibition Test for the Detection of Antimicrobial Residues in Living Animals Intended for Human Consumption. Foods. 2021; 10(8):1897. https://doi.org/10.3390/foods10081897

Chicago/Turabian Style

Serrano, María Jesús, Luis Mata, Diego García-Gonzalo, Alejandra Antón, Pedro Razquin, Santiago Condón, and Rafael Pagán. 2021. "Optimization and Validation of a New Microbial Inhibition Test for the Detection of Antimicrobial Residues in Living Animals Intended for Human Consumption" Foods 10, no. 8: 1897. https://doi.org/10.3390/foods10081897

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop