Novel Antioxidant and Hypoglycemic Water-Soluble Polysaccharides from Jasmine Tea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Preparation of Jasmine Tea
2.3. Extraction, Isolation, and Purification of Crude Polysaccharides from Jasmine Tea
2.4. Functional Activities of JSP Sub-Fractions
2.4.1. Antioxidant Activities
2.4.2. Hypoglycemic Effect
Cell Culture
Cytotoxicity Assay
Protective Effect on High-Glucose-Induced RIN-m5F Cell Injury
2.5. Primary Structure Analysis of JSP Sub-Fractions
2.5.1. Determination of Molecular Weight and Homogeneity
2.5.2. Analysis of Monosaccharide Composition
2.5.3. FTIR and UV Analysis
2.5.4. Congo Red Analysis
2.5.5. Analysis of NMR Spectroscopy
2.6. Statistical Analysis
3. Results and Discussion
3.1. Isolation and Purification of Crude JSP
3.2. Functional Activities of JSP Sub-Fractions
3.2.1. Antioxidant Activities
3.2.2. Hypoglycemic Activities
3.3. Structural Analysis of JSP Sub-Fractions
3.3.1. Monosaccharide Composition and Molecular Weight
3.3.2. FTIR and UV Analysis
3.3.3. Congo Red Test
3.3.4. NMR Studies
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Sample | Molecular Weight (kDa) | Monosaccharide Composition (Molar Ratio) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Man | Glu | Gal | Rha | Fuc | Xyl | Ara | GlcA | GalA | ||
JSP-1 | 18.4 | 0.06 | 1.14 | 4.69 | 1.00 | 0.18 | 9.92 | 13.79 | 0.10 | 4.09 |
JSP-2 | 14.1 | 0.29 | - | 3.80 | 1.00 | 0.17 | 8.27 | 11.85 | 0.14 | 5.05 |
Sugar Residues | 1 | 2 | 3 | 4 | 5 | 6 | ||
---|---|---|---|---|---|---|---|---|
A1 | 1,3-linked α-Galf | H | 5.36 | 3.61 | 3.93 | 4.10 | 3.69 | 3.81 |
C | 99.75 | 71.64 | 76.75 | 80.87 | 72.85 | 60.66 | ||
A2 | 1,2-linked α-Galf | H | 5.36 | 3.63 | 3.93 | 4.19 | 3.80 | 3.69 |
C | 99.78 | 77.07 | 73.39 | 81.79 | 71.21 | 61.43 | ||
B | 1,5-linked α-Araf | H | 5.06 | 4.10 | 3.99 | 4.19 | 3.85 | - |
C | 107.54 | 80.96 | 76.55 | 82.25 | 67.60 | - | ||
C | 1,3,5-linked α-Araf | H | 5.12 | - | 4.26 | 4.06 | 3.90 | - |
C | 107.23 | - | 84.21 | 79.44 | 69.75 | - | ||
D | 1,3-linked α-Araf | H | 5.21 | 4.19 | 4.10 | 4.24 | - | - |
C | 109.27 | 80.88 | 84.12 | 81.36 | - | - | ||
E | T-linked α-Araf | H | 5.08 | 4.27 | 4.19 | 4.00 | 3.69 | - |
C | 107.57 | 81.32 | 81.52 | 83.70 | 61.38 | - | ||
F | 1,2-linked β-Xylp | H | 4.51 | 3.62 | 3.70 | 3.39 | 3.84 | - |
C | 103.26 | 71.44 | 75.23 | 69.46 | 60.66 | - | ||
G | 1,3-linked β-Xylp | H | 4.62 | 3.63 | 3.75 | 4.07 | 3.84 | - |
C | 104.42 | 77.23 | 80.01 | 74.54 | 60.66 | - |
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Tang, Y.; Sheng, J.; He, X.; Sun, J.; Wei, Z.; Liu, G.; Li, C.; Lin, B.; Li, L. Novel Antioxidant and Hypoglycemic Water-Soluble Polysaccharides from Jasmine Tea. Foods 2021, 10, 2375. https://doi.org/10.3390/foods10102375
Tang Y, Sheng J, He X, Sun J, Wei Z, Liu G, Li C, Lin B, Li L. Novel Antioxidant and Hypoglycemic Water-Soluble Polysaccharides from Jasmine Tea. Foods. 2021; 10(10):2375. https://doi.org/10.3390/foods10102375
Chicago/Turabian StyleTang, Yayuan, Jinfeng Sheng, Xuemei He, Jian Sun, Zhen Wei, Guoming Liu, Changbao Li, Bo Lin, and Li Li. 2021. "Novel Antioxidant and Hypoglycemic Water-Soluble Polysaccharides from Jasmine Tea" Foods 10, no. 10: 2375. https://doi.org/10.3390/foods10102375