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Biofunctionalized Polyelectrolyte Microcapsules Encoded with Fluorescent Semiconductor Nanocrystals for Highly Specific Targeting and Imaging of Cancer Cells

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Laboratory of Nano-Bioengineering, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 31 Kashirskoe shosse, Moscow 115409, Russian
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Laboratoire de Recherche en Nanosciences, LRN-EA4682, Universite de Reims Champagne-Ardenne, 51 rue Cognacq Jay, Reims 51096, France
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N.N. Blokhin National Medical Research Center of Oncology, 23 Kashirskoe shosse, Moscow 115478, Russian
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Translational Molecular Imaging, Max-Planck-Institute of Experimental Medicine, 3 Hermann-Rein-Str., Göttingen 37075, Germany
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Clinic of Haematology and Medical Oncology, University Medical Center Göttingen, 40 Robert-Koch-Str., Göttingen 37075, Germany
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Department of Clinical Immunology and Allergology, I.M. Sechenov First Moscow State Medical University, Moscow 119992, Russian
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Authors to whom correspondence should be addressed.
This paper is an extended version of Nifontova, G.; Baryshnikova, M.; Ramos Gomes, F.; Alves, F.; Nabiev, I.; Sukhanova, A. Engineering of fluorescent biomaging tools for cancer cell targeting based on polyelectrolyte microcapsules encoded with quantum dots. In Proceedings of the 4th International Conference on Applications of Optics and Photonics, AOP2019, Lisbon, Portugal, 31 May–1 June 2019.
Photonics 2019, 6(4), 117; https://doi.org/10.3390/photonics6040117 (registering DOI)
Received: 25 September 2019 / Revised: 31 October 2019 / Accepted: 5 November 2019 / Published: 8 November 2019
Fluorescent semiconductor nanocrystals or quantum dots (QDs) are characterized by unique optical properties, including a high photostability, wide absorption spectrum, and narrow, symmetric fluorescence spectrum. This makes them attractive fluorescent nanolabels for the optical encoding of microcarriers intended for targeted drug delivery, diagnosis, and imaging of transport processes on the body, cellular, and subcellular levels. Incorporation of QDs into carriers in the form of polyelectrolyte microcapsules through layer-by-layer adsorption of oppositely charged polyelectrolyte polymers yields microcapsules with a bright fluorescence signal and adaptable size, structure, and surface characteristics without using organic solvents. The easily modifiable surface of the microcapsules allows for its subsequent functionalization with capture molecules, such as antibodies, which ensures specific and selective interaction with cells, including tumor cells, with the use of the bioconjugation technique developed here. We obtained stable water-soluble nanolabels based on QDs whose surface was modified with polyethylene glycol (PEG) derivatives and determined their colloidal and optical characteristics. The obtained nanocrystals were used to encode polyelectrolyte microcapsules optically. The microcapsule surface was modified with humanized monoclonal antibodies (Abs) recognizing a cancer marker, epidermal growth factor receptor (EGFR). The possibility of effective, specific, and selective delivery of the microcapsules to tumor cells expressing EGFR has been demonstrated. The results show that the QD-encoded polyelectrolyte microcapsules functionalized with monoclonal Abs against EGFR can be used for targeted imaging and diagnosis. View Full-Text
Keywords: quantum dots; fluorescent imaging; fluorescent nanolabels; polyelectrolyte microcapsules; antibody-mediated targeted delivery quantum dots; fluorescent imaging; fluorescent nanolabels; polyelectrolyte microcapsules; antibody-mediated targeted delivery
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Nifontova, G.; Kalenichenko, D.; Baryshnikova, M.; Ramos Gomes, F.; Alves, F.; Karaulov, A.; Nabiev, I.; Sukhanova, A. Biofunctionalized Polyelectrolyte Microcapsules Encoded with Fluorescent Semiconductor Nanocrystals for Highly Specific Targeting and Imaging of Cancer Cells. Photonics 2019, 6, 117.

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