Combination of Medium- and High-Pressure Liquid Chromatography for Isolation of L-tryptophan (Q-marker) from Medicago sativa Extract
Abstract
:1. Introduction
2. Materials and Methods
2.1. Equipment and Chemicals
2.2. Preparation of Extract of Medicago Sativa
2.3. Medium-Pressure Liquid Chromatography Sample Pretreatment
2.4. High-Pressure Liquid Chromatography Purification Quality Marker
2.5. Purity Evaluation of the Obtained Quality Marker
3. Results
3.1. Medium-Pressure Chromatography for Enrichment of the Quality Marker Faction
3.2. High-Pressure Preparation and Purity Analysis of the Isolated Quality Marker
3.3. Structural Characterization of the Isolated Quality Marker
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Zhang, S.; Ma, Y.; Ma, R.; Wang, Q.; Dang, J. Combination of Medium- and High-Pressure Liquid Chromatography for Isolation of L-tryptophan (Q-marker) from Medicago sativa Extract. Separations 2022, 9, 240. https://doi.org/10.3390/separations9090240
Zhang S, Ma Y, Ma R, Wang Q, Dang J. Combination of Medium- and High-Pressure Liquid Chromatography for Isolation of L-tryptophan (Q-marker) from Medicago sativa Extract. Separations. 2022; 9(9):240. https://doi.org/10.3390/separations9090240
Chicago/Turabian StyleZhang, Shixuan, Yonggui Ma, Ronghua Ma, Qilan Wang, and Jun Dang. 2022. "Combination of Medium- and High-Pressure Liquid Chromatography for Isolation of L-tryptophan (Q-marker) from Medicago sativa Extract" Separations 9, no. 9: 240. https://doi.org/10.3390/separations9090240