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Article

Extraction, Quantification, and Cytokine Inhibitory Response of Bakuchiol in Psoralea coryfolia Linn.

1
Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110062, India
2
Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110062, India
3
Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
4
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
*
Author to whom correspondence should be addressed.
Separations 2020, 7(3), 48; https://doi.org/10.3390/separations7030048
Received: 18 August 2020 / Revised: 27 August 2020 / Accepted: 31 August 2020 / Published: 11 September 2020
(This article belongs to the Special Issue Extraction and Isolation of Natural Products)
(1) Background: The present investigation studies the optimization of extraction, quantification, and cytokine inhibitory effects bakuchiol (BKL) in Psoralea coryfolia Linn. (2) Methods: The seeds of Psoralea coryfolia cleaned, dried, and powdered. Different separation methods maceration, reflux, Soxhlet, and ultrasonic assisted extraction (UAE) were employed for the isolation of BKL by five pure solvents. The quantity of BKL was measured by high-performance liquid chromatography (HPLC) method to determine the highest yield percentage. The effect of optimized BKL was then tested in an animal model of sepsis induced by lipopolysaccharides (LPS). (3) Results: The UAE method was found to be the best among tested separation methods and yielded highest percentage of BKL in petroleum ether extract. Septic rats showed a significant elevation in levels of biochemical markers like AST, ALT, ALP, BIL, SCr, and BUN in plasma. Proinflammatory cytokines (TNF-α and IL-1) levels were also increased in LPS-induced animals. BKL has been found to significantly reverse these elevated levels as compared to the LPS-induced animals. (4) Conclusion: The present results suggest that BKL has positive effects when administered in animals with pathogenic shock by decreasing the circulating levels of biomarkers. Further studies are necessary to explore the clinical implications of such findings. View Full-Text
Keywords: Psoralea coryfolia; extraction; bakuchiol; HPLC; LPS; cytokine Psoralea coryfolia; extraction; bakuchiol; HPLC; LPS; cytokine
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MDPI and ACS Style

Khuranna, D.; Sharma, S.; Mir, S.R.; Aqil, M.; Ahmad, A.; Rehman, M.U.; Ahmad, P.; Alwahibi, M.S.; Elshikh, M.S.; Mujeeb, M. Extraction, Quantification, and Cytokine Inhibitory Response of Bakuchiol in Psoralea coryfolia Linn. Separations 2020, 7, 48. https://doi.org/10.3390/separations7030048

AMA Style

Khuranna D, Sharma S, Mir SR, Aqil M, Ahmad A, Rehman MU, Ahmad P, Alwahibi MS, Elshikh MS, Mujeeb M. Extraction, Quantification, and Cytokine Inhibitory Response of Bakuchiol in Psoralea coryfolia Linn. Separations. 2020; 7(3):48. https://doi.org/10.3390/separations7030048

Chicago/Turabian Style

Khuranna, Deepak, Sanchit Sharma, Showkat R. Mir, Mohd Aqil, Ajaz Ahmad, Muneeb U. Rehman, Parvaiz Ahmad, Mona S. Alwahibi, Mohamed S. Elshikh, and Mohd Mujeeb. 2020. "Extraction, Quantification, and Cytokine Inhibitory Response of Bakuchiol in Psoralea coryfolia Linn." Separations 7, no. 3: 48. https://doi.org/10.3390/separations7030048

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