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Volume 11
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10.3390/separations11050140
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Article

Development and Validation of an LC-MS/MS Method for the Determination of Plasma and Red Blood Cell Omega Fatty Acids: A Useful Diagnostic Tool

by
Lénárd Farczádi
1,
Minodora Dobreanu
1,2,*,
Adina Huțanu
1,2 and
Silvia Imre
1,3
1
Center of Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540142 Targu Mures, Romania
2
Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540142 Targu Mures, Romania
3
Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540142 Targu Mures, Romania
*
Author to whom correspondence should be addressed.
Separations 2024, 11(5), 140; https://doi.org/10.3390/separations11050140
Submission received: 18 March 2024 / Revised: 12 April 2024 / Accepted: 26 April 2024 / Published: 1 May 2024
(This article belongs to the Special Issue Analysis and Bioanalysis of Pharmaceuticals: Sample Preparation and Chromatography)
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Abstract

Background: LC-MS is an ever-increasingly used methodology for clinical applications. Due to the superior selectivity and sensitivity, in certain situations, it can offer an advantage or be the only option for diagnostics and biomonitoring applications. Methods: A high selectivity sensitive LC-MS/MS method was developed for direct quantification of free plasma polyunsaturated fatty acids as well as conjugated membrane polyunsaturated fatty acids, using isocratic reverse phase elution. A quick and simple sample purification method was used in order to ensure high-throughput analysis of biological samples. The method was validated with regard to selectivity, sensitivity, linearity, accuracy, precision, carryover, and recovery, as well as other relevant parameters. Results and Conclusions: The method was developed and validated with respect to all relevant parameters and was successfully used in a number of clinical diagnostics and biomonitoring applications. The simple sample purification process allowed for an easy learning curve for analysts and other users, while ensuring a low chance of systematic or random errors and thus reliable results usable in a clinical setting.
Keywords: PUFA; LC-MS; metabolomics; diagnostic PUFA; LC-MS; metabolomics; diagnostic

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MDPI and ACS Style

Farczádi, L.; Dobreanu, M.; Huțanu, A.; Imre, S. Development and Validation of an LC-MS/MS Method for the Determination of Plasma and Red Blood Cell Omega Fatty Acids: A Useful Diagnostic Tool. Separations 2024, 11, 140. https://doi.org/10.3390/separations11050140

AMA Style

Farczádi L, Dobreanu M, Huțanu A, Imre S. Development and Validation of an LC-MS/MS Method for the Determination of Plasma and Red Blood Cell Omega Fatty Acids: A Useful Diagnostic Tool. Separations. 2024; 11(5):140. https://doi.org/10.3390/separations11050140

Chicago/Turabian Style

Farczádi, Lénárd, Minodora Dobreanu, Adina Huțanu, and Silvia Imre. 2024. "Development and Validation of an LC-MS/MS Method for the Determination of Plasma and Red Blood Cell Omega Fatty Acids: A Useful Diagnostic Tool" Separations 11, no. 5: 140. https://doi.org/10.3390/separations11050140

APA Style

Farczádi, L., Dobreanu, M., Huțanu, A., & Imre, S. (2024). Development and Validation of an LC-MS/MS Method for the Determination of Plasma and Red Blood Cell Omega Fatty Acids: A Useful Diagnostic Tool. Separations, 11(5), 140. https://doi.org/10.3390/separations11050140

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