3.1. Light Field and L/D Boundary
When the single-sided light is incident to the PBR along the +
x-axis (
Figure 4), the light path
, where (
x,
y) are the coordinates of a point in the tube, and
r is the reactor radius (m). It is assumed that the incident light intensity
I0 = 800 μmol m
−2 s
−1 [
45]. The light field on the cross section of the reactor can be obtained according to the light model shown in
Section 2.3 (
Figure 4b). The white line in the figure represents the L/D boundary, which is the position where
Ic = 96.84 μmol m
−2 s
−1.
When the double-sided light is incident (
Figure 4c), the light intensity at a certain point on the cross section cannot be calculated using the light path of only one side. However, because the light intensity is a scalar, the light intensity at a point equals the algebraic sum of the light intensities at this point when the individual light on each side is incident separately. Since the cross-sectional area of the spiral ribs does not exceed 2.8% of the total cross-sectional area, the influence of the spiral rib interface on the light field can be ignored. The total light intensity of the double-sided illumination is assumed to be the same as that of the single-sided illumination. Hence, the intensity of the incident light on each side is
I0 = 400 μmol m
−2 s
−1. The light field on the cross section of the reactor can be obtained according to the light model presented in
Section 2.3 (
Figure 4d). The white line represents the L/D boundary. Compared with the L/D boundary under the single-sided illumination conditions (
Figure 4b), the L/D boundary under the double-sided illumination conditions moves toward the tube wall, and the curvature of the L/D boundary decreases, but the length of the L/D boundary increases.
3.2. Optimization of a PBR with Spiral Ribs under Single-Sided Illumination
The structural parameters of the spiral ribs and the flow rate of the algal suspension are the main factors affecting the L/D cycle performance of the algae cells. The main structural parameters of the spiral ribs include the number of spiral ribs (N), the inclination angle of the spiral ribs (α (°)), and the pitch of the spiral ribs (P (mm)). However, P and α are not independent of each other because of the linear geometric relationship between them. For example, a reactor with P = 200 mm has a spiral rib inclination angle α = 38.15°; a reactor with α = 60° has a spiral rib pitch P = 90.69 mm. Therefore, in this study, N, α, and uin were selected as influencing factors to obtain the best structural and operating parameters for the optimization of the spiral rib PBR.
The orthogonal design method was adopted to reduce the computational load and save calculation time. The factor-level table is shown in
Table 1. In this study, five levels of 1, 2, 3, 4, and 5 are set for
N (i.e., factor A), four levels of 15°, 30°, 45°, and 60° are set for
α (i.e., factor B), and three levels of 0.4, 0.5, and 0.6 m s
−1 are set for
uin (i.e., factor C). Compared to the full test, which requires a total of 5 × 4 × 3 = 60 tests, the orthogonal design only requires 25 tests. The combination of factors is shown in
Table 2, where the subscript represents the level of factor A, B or C. For instance,
A1B4C2 represents that
N is in level 1, α is in level 4, and
uin is in level 2, i.e.,
N = 1, α = 60° and
uin = 0.5 m s
−1. The simulation analysis was carried out on the 25 test groups in
Table 2. The average L/D cycle frequency (
fav) and the efficiency of the L/D cycle enhancement (
η) for each group were obtained. The data were evaluated using data analysis software, and the results are shown in
Table 3.
When performing the analysis of variance (ANOVA) on
fav and
η, the validity of the experimental data was first determined using the significance test. When the significance level is less than 0.05, the difference between the data sets can be considered statistically significant; i.e., the factor has a significant effect on the dependent variable. According to
Table 3, the significance levels of
N,
α, and
uin for both performance indicators (
fav and
η) are all less than 0.05, which indicates that the effects of the three factors on the two performance indicators are significant.
F refers to the F-test, which is the overall significance test. When the significance level is less than 0.05, the influence of the factor on the performance indicator can be directly evaluated by the value of F. The larger F is, the more pronounced the influence is. The F values for
fav are shown in
Table 3. It is obvious that F(
α) >> F(
N) ≅ (
uin), it means that
α has much greater influence on
fav compared to
N and
uin. Similarly, the influences of the three factors on
η follow the order F(
N) > F(
α) > F(
uin).
To visualize the relationships between the three factors and the two performance indicators, the average value of
fav and
η at each test level was calculated and plotted in
Figure 5 and
Figure 6, respectively.
Figure 5 shows that as
N increases,
fav first decreases and then increases. The mean of
fav is the lowest with 4 spiral ribs. Increasing
α can significantly increase
fav. In addition,
fav reaches its maximum when
uin is 0.5 m s
−1. The trends of the three plots show that
α has the greatest influence on
fav, which is consistent with the results determined based on the significance levels and the F values. Among the three plots, the optimal levels of the three factors (i.e.,
N = 1,
α = 60°, and
uin = 0.5 m s
−1) correspond to the maximum values of
fav. The combination of the optimal levels is group 1 in
Table 2. With this combination,
fav is 2.28 Hz, which is the highest of all 25 tests.
Figure 6 shows the relationships between
η and the three factors. When using
η as a performance indicator, the optimal
N is 1, the optimal
α is 30°, and the optimal
uin is 0.4 m s
−1. An analysis of the trends of the three plots shows that
N has the greatest influence on
η, while
uin has the smallest influence on
η. This outcome is consistent with the results determined based on the significance levels and the F values. The optimal combination of parameters corresponds to group 24 in
Table 2. With this combination,
η is 2.01, which is the highest of all 25 tests.
The concentration of microalgae is directly related to
fav. Studies have shown that the higher
fav is (below 100 Hz), the better the growth of microalgae is [
16]. Of the two optimal combinations shown above, group 1 has
fav of 2.28 Hz and
η of 1.22, whereas group 24 has
fav of 0.75 Hz and
η of 2.01.
fav of group 1 is much higher than that of group 24. Moreover,
η of group 1 is greater than 1, which indicates that the increase in the energy consumption of group 1 is less than the increase in
fav. This outcome is desirable from an economic point of view because the benefit obtained is greater than the cost. Therefore, the optimal parameter combination for the spiral rib tubular PBR under single-sided illumination is the combination in group 1, which has the following parameters:
N = 1,
α = 60°, and
uin = 0.5 m s
−1.
3.3. Optimization of the PBR with Spiral Ribs under Double-Sided Illumination
The orthogonal experiment was carried out on the tubular PBR under double-sided illumination. The influencing factors and their levels are the same as those used in the single-sided case, and the orthogonal table is the same as
Table 2. The test results are shown in
Table 4. Based on the significance levels and the F values, all three factors have significant effects on the L/D cycle performance under double-sided illumination. For
fav, the influences of the three factors follow the order F(
α) >> F(
N) ≅ F(
uin). For
η, the influences of the three factors follows the order F(
α) > F(
uin) > F(
N).
The relationships between the three factors and the two performance indicators (
fav and
η) are shown in
Figure 7 and
Figure 8, respectively.
Figure 7 shows that as
N increases,
fav first decreases and then increases. The mean of
fav is the lowest with 4 spiral ribs. Increasing
α can significantly increase
fav. In addition,
fav reaches the maximum when
uin is 0.6 m/s. The trends of the three plots show that compared to
N and
uin,
α has much greater influence on
fav. This outcome is consistent with the results determined based on the significance levels and the F values.
Based on the trends of the three plots, the optimal combination of the three factors is
N = 1,
α = 60°, and
uin = 0.6 m s
−1. However, this combination is not included in the 25 combinations in
Table 2. Therefore, it is necessary to remodel and recalculate the results.
fav of this combination is calculated to be 2.36 Hz, which is higher than those of the 25 test groups.
η of this combination is 0.60.
The relationships between
η and the three factors (
Figure 8) show that when
η is used as the performance indicator, the optimal
N is 5, the optimal
α is 45°, and the optimum
uin is 0.5 m s
−1. This combination is group 21 in
Table 2, and the corresponding
η is 0.142. However, further verification shows that
η of group 21 is not the highest of the 25 test groups. The efficiency of group 11 is the highest (0.53) of all 25 test groups but is still less than that of the combination of
N = 1,
α = 60°, and
uin = 0.6 m s
−1 (0.60). This outcome may occur because of the interactions between various factors, indicating that in the case of double-sided illumination, the effects of the three influencing factors are non-independent and need to be more comprehensively understood. This indication should be studied further in the future. In summary, in the case of double-sided illumination, the optimal parameter combination is
N = 1,
α = 60°, and
uin = 0.6 m s
−1.
It is worth noting that the efficiencies of the L/D cycle enhancement are negative in all cases except for three rib inclination angles (
Figure 8). Based on the formula for
η (Equation (17)), there are two possible reasons for these negative values: one is
fav <
fav,0, and the other is
. A comparison of
fav between the spiral rib PBRs and the plain PBR shows that the first reason (
fav <
fav,0) causes the negative efficiency. Under double-sided illumination,
fav of the spiral rib PBR is less than that of a plain PBR under the same conditions. This result shows that the higher pumping cost does not lead to an increase in
fav. Therefore, the negative efficiency of the L/D cycle enhancement is not desirable for optimizing the L/D cycle performance of the algae.
3.4. Comparison between Single-Sided and Double-Sided Illuminations and Analysis of Causes
The light field (
Figure 4) shows that the L/D boundary under single-sided illumination is located only on one side of the flow field (
Figure 4b), while the L/D boundary under double-sided illumination is longer and has a circular form (
Figure 4d). Intuitively, a long L/D boundary can increase the probability and the number of particles participating in the L/D cycles. As a result,
fav of particles under double-sided illumination should be greater than that under single-sided illumination. However, the results of the orthogonal test show that the average L/D cycle frequencies under single-sided illumination with
I0 = 800 μmol m
−2 s
−1 are generally higher than those under double-sided illumination with
I0 = 400 μmol m
−2 s
−1. Therefore, for a tubular PBR with spiral ribs, the use of double-sided illumination does not increase
fav. In addition, under double-sided illumination, the efficiencies of the L/D cycle enhancement in the 25 tests are all less than 1 and are sometimes less than 0, which means that the increase in the pumping cost per unit time caused by the spiral ribs is greater than the increase in the L/D cycle frequency caused by the ribs. In other words, a higher pumping cost does not result in an increase in the L/D cycle frequency. The reason why double-sided illumination does not increase
fav of microalgae particles can be discussed from the following three perspectives: the number of particles participating in the L/D cycles, the probability distribution of the L/D cycle frequency of the particles, and the relative position between the vortex and the L/D boundary in the reactor.
A comparison of the orthogonal test results under single-sided and double-sided illumination conditions shows that the differences in
fav are large in tests 2, 5, 7, 13, 14, and 22. The corresponding average L/D cycle frequencies and the numbers of particles participating in the L/D cycles are shown in
Table 5. In these six tests (including single-sided illumination and double-sided illumination), between 60% and 69.5% of the 1200 particles released are involved in the L/D cycles. The other particles do not participate in the L/D cycles; they are either always in the light zone or always in the dark zone but do not cross the L/D boundary during the time they are being tracked. The results show that
fav of the particles under double-sided illumination is 15.1–24.0% lower than that under single-sided illumination. The total number of particles participating in the L/D cycles under double-sided illumination is reduced by 15.1–33.2% compared with that under single-sided illumination. Therefore, one of the reasons for the low average L/D cycle frequency under double-sided illumination is that fewer particles participate in the L/D cycle.
The probability density is plotted against
fav under different illumination conditions for tests 2, 5, 7, 13, 14, and 22 (
Figure 9). In each of these tests, the average L/D cycle frequencies of some particles increase significantly under double-sided illumination compared with that under single-sided illumination. For example, in tests 2, 5, 7, 13, and 22, the average L/D cycle frequencies of some particles can reach 6–7 Hz under double-sided illumination. However, such particles only account for a small proportion of the probability density.
Taking test 22 as an example, fav of the particles under single-sided illumination ranges from 0 to 4 Hz. The range of the average L/D cycle frequencies is greater under double-sided illumination, which is 0–6.5 Hz. However, the probability density of the frequencies in the range of 4–6.5 Hz is very low. Under single-sided illumination, 47% of the 1200 released particles experience L/D cycles with frequencies of 2–4 Hz. Under double-sided illumination, this percentage is only 25%. This result shows that under double-sided illumination, the percentage of particles participating in high-frequency L/D cycles decreases. This outcome is another reason that fav of the particles under double-sided illumination is generally low.
The discussion presented above shows that fav of the particles is closely related to two factors: the number of particles participating in the L/D cycles and the probability distribution of the L/D cycle frequency of the particles. The relative position between the vortex and the L/D boundary in the reactor is the root cause of these two factors. fav is analyzed in the following section from the perspectives of the flow field and the L/D boundary.
The pressure distributions, streamlines, and L/D boundaries on the cross section in the center of the
L segment (i.e., the cross section at z = 2.5 m) under different illumination modes for tests 2, 5, 7, 13, 14, and 22 are shown in
Figure 10 and
Figure 11. The flow field can be roughly divided into three concentric regions: the inner region (0 <
r0 < 0.008 m), the middle region (0.008 <
r0 < 0.016 m), and the outer region (0.016 <
r0 < 0.025 m). The boundaries between the three regions are shown in red. The white dotted line in
Figure 10 is the L/D boundary in the case of single-sided illumination, and the blue dotted line in
Figure 11 is the L/D boundary in the case of double-sided illumination. The centers of the large vortices in these six tests are generally located in the inner region. In the case of single-sided illumination, the L/D boundaries pass through both the middle region and the outer region; in the case of double-sided illumination, the L/D boundaries are in the outer region and are annular.
The radial position of the particle affects its L/D cycle performance. The changes in radial position of the particles initially in the inner, middle and outer regions at the inlet section of the
L segment (i.e., the cross section at z = 2 m) in the six tests was studied. The results show that the movements of particles initially in the inner region are radially concentrated in the inner and middle regions, the movements of particles initially in the middle region spread across the inner, middle and outer regions, and the movements of particles initially in the outer region are radially concentrated in the outer and middle regions.
Figure 12 shows the changes in the radial positions of the particles in test 2 and test 5. The L/D cycle performances of the particles in three different initial regions in the six tests were statistically studied in combination with the L/D boundary. Under single-sided illumination, 11.2–16.3% of the 1200 released particles initially in the inner region participated in the L/D cycles, 28.3–31.1% of the particles initially in the middle region participated in the L/D cycles, and 20.7–28.1% of the particles initially in the outer region participated in the L/D cycles. Similarly, under double-sided illumination, the three percentages are 7.0–10.9%, 18.1–24.6%, and 15.6%–26.5%, respectively. When single-sided illumination is switched to double-sided illumination, the numbers of particles undergoing L/D cycles are all significantly reduced regardless of their initial positions. These results show that splitting single-sided illumination into two illuminations on both sides cannot increase the number of particles undergoing the L/D cycles when using the reactor structure employed in this study.
fav of the particles in the six tests was statistically studied based on the initial positions of the particles. The statistical results are shown in
Table 6. Under double-sided illumination, the average L/D cycle frequencies of the particles are all lower regardless of their initial positions. Combined with the influence of the L/D boundary on the number of particles participating in the L/D cycles, the results show that the reason for the decrease in
fav is that the change in the position of the L/D boundary not only reduces the number of particles undergoing the L/D cycles but also decreases the L/D cycle frequencies of the particles in the different regions.
3.5. Development and Validation of a Method for Increasing the Average L/D Cycle Frequency
The flow field determines the radial position of the particles, and the light field determines the position of the L/D boundary. The phenomenon in which particles participate in the L/D cycles is a result of the synergy between the flow field and the light field. Therefore, two methods could be considered to increase the number of particles participating in the L/D cycles and the percentage of particles participating in the high-frequency L/D cycles, thereby increasing fav of the particles: changing the flow field (the vorticity field) by changing the structure of the reactor so that more particles can pass through the L/D boundary and changing the position of the L/D boundary based on the radial position of the particles in the flow field. The second method is validated below.
It was shown in
Section 3.4 that the movements of particles initially in the inner region are radially concentrated in the inner and middle regions, and the movements of particles initially in the outer region are radially concentrated in the middle and outer regions. The L/D boundaries discussed above under different illumination modes do not pass through the inner regions, as shown in
Figure 10 and
Figure 11. Therefore, it is assumed that the L/D boundary can pass through the three regions simultaneously by adjusting the light intensity and the incident direction, as shown in
Figure 13, where the white line is the L/D boundary.
fav under the conditions shown in
Figure 13 was calculated for the 25 test groups in the orthogonal test. The result is compared with that obtained under single-sided illumination conditions (
Figure 14). Adjusting the position and curvature of the L/D boundary according to the flow field increases
fav of the particles. This increase occurs because the change in the L/D boundary increases the number of particles undergoing the L/D cycles, and the change in the L/D boundary can simultaneously increase the percentage of particles involved in the high-frequency L/D cycles. Taking group 6, which has the largest frequency increase, as an example, the number of particles participating in the L/D cycles under the conditions shown in
Figure 13 is 59.1% higher than that under the single-sided illumination conditions. The probability density function (
Figure 15) shows that the L/D cycle frequencies of most of the particles is near 1 Hz under the conditions shown in
Figure 13. The percentage of particles participating in the high-frequency L/D cycles under these conditions is significantly higher than that under single-sided illumination. The increase in
fav of the optimal combination under single-sided illumination (group 1 in
Table 2) is the smallest (only 26.3%). These results show that in the case of single-sided illumination, the synergy between the flow field and the light field of group 1 is good.