Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions

Isoprenoids are among the most abundant biogenic volatile compounds (VOCs) emitted by plants, and mediate both biotic and abiotic stress responses. Here, we provide for the first time a comparative analysis of transgenic Arabidopsis lines constitutively emitting isoprene and ocimene. Transgenic lines and Columbia-0 (Col-0) Arabidopsis were characterized under optimal, water stress, and heat stress conditions. Under optimal conditions, the projected leaf area (PLA), relative growth rate, and final dry weight were generally higher in transgenics than Col-0. These traits were associated to a larger photosynthetic capacity and CO₂ assimilation rate at saturating light. Isoprene and ocimene emitters displayed a moderately higher stress tolerance than Col-0, showing higher PLA and gas-exchange traits throughout the experiments. Contrasting behaviors were recorded for the two overexpressors under water stress, with isoprene emitters showing earlier stomatal closure (conservative behavior) than ocimene emitters (non-conservative behavior), which might suggest different induced strategies for water conservation and stress adaptation. Our work indicates that (i) isoprene and ocimene emitters resulted in enhanced PLA and biomass under optimal and control conditions and that (ii) a moderate stress tolerance is induced when isoprene and ocimene are constitutively emitted in Arabidopsis, thus providing evidence of their role as a potential preferable trait for crop improvement.