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Open AccessArticle

Effects of Monoterpenes of Trachyspermum ammi on the Viability of Spermatogonia Stem Cells In Vitro

1
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, 1417614411 Tehran, Iran
2
Infertility & Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, 1417614411 Tehran, Iran
3
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, 1417614411 Tehran, Iran
4
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, 1417614411 Tehran, Iran
*
Author to whom correspondence should be addressed.
Plants 2020, 9(3), 343; https://doi.org/10.3390/plants9030343
Received: 8 February 2020 / Revised: 27 February 2020 / Accepted: 28 February 2020 / Published: 9 March 2020
(This article belongs to the Special Issue Mechanisms of Plant Antioxidants Action)
Trachyspermum ammi (Apiaceae) plants have several medicinal and condimentary applications and are considered an aphrodisiac agent in Iranian Traditional Medicine. Thus, the present study aims to evaluate the effects of oil from Iranian T. ammi plants on the viability of spermatogonial stem cells in vitro. The essential oil of T. ammi fruits was extracted by hydrodistillation, and the amount of thymol was calculated by a gas-chromatography method. Spermatogonial stem cells were isolated from the testes of mice using enzyme digestion. Real-time polymerase chain reaction (RT-PCR) was applied to assess the gene expressions of promyelocytic leukemia zinc finger protein (Plzf), DNA-binding protein inhibitor (ID-4), tyrosine-protein kinase (c-Kit), B-cell lymphoma 2 (Bcl2) and Bcl2-associated X protein (BAX). The number and diameter of colonies were also measured in the treated cells. The amount of thymol in the oil was 130.7 ± 7.6 µg/mL. Flow cytometry analysis showed that 92.8% of all cells expressed stimulated by retinoic acid 8 (Stra8), a spermatogonial stem cell marker. Expression of Plzf and ID-4 genes significantly increased in the treatment groups, while c-Kit and BAX decreased, and Bcl2 increased in the presence of essential oil. The numbers and diameters of cells were also improved by the application of the plant oil. These data indicated that monoterpenes from the oil of T. ammi improved the quality and viability of spermatogonia cells in the cell culture. View Full-Text
Keywords: apiaceae; stem cells; T. ammi; thymol apiaceae; stem cells; T. ammi; thymol
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MDPI and ACS Style

Omidpanah, S.; Aliakbari, F.; Nabavi, S.M.; Vazirian, M.; Hadjiakhoondi, A.; Kurepaz-mahmoodabadi, M.; Manayi, A. Effects of Monoterpenes of Trachyspermum ammi on the Viability of Spermatogonia Stem Cells In Vitro. Plants 2020, 9, 343.

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