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Article

Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome

1
Department of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech Republic
2
Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, 77200 Olomouc, Czech Republic
3
Department of Biological Sciences, Auburn University, Auburn, AL 811, USA
4
Central European Institute of Technology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Present address Agricultural Research, Ltd., Zahradní 1, 664 41 Troubsko, Czech Republic.
Plants 2020, 9(11), 1563; https://doi.org/10.3390/plants9111563
Received: 26 October 2020 / Revised: 10 November 2020 / Accepted: 11 November 2020 / Published: 13 November 2020
Cytokinins are multifaceted plant hormones that play crucial roles in plant interactions with the environment. Modulations in cytokinin metabolism and signaling have been successfully used for elevating plant tolerance to biotic and abiotic stressors. Here, we analyzed Arabidopsis thaliana response to INhibitor of CYtokinin DEgradation (INCYDE), a potent inhibitor of cytokinin dehydrogenase. We found that at low nanomolar concentration, the effect of INCYCE on seedling growth and development was not significantly different from that of trans-Zeatin treatment. However, an alteration in the spatial distribution of cytokinin signaling was found at low micromolar concentrations, and proteomics analysis revealed a significant impact on the molecular level. An in-depth proteome analysis of an early (24 h) response and a dose-dependent response after 168 h highlighted the effects on primary and secondary metabolism, including alterations in ribosomal subunits, RNA metabolism, modulations of proteins associated with chromatin, and the flavonoid and phenylpropanoid biosynthetic pathway. The observed attenuation in stress-response mechanisms, including abscisic acid signaling and the metabolism of jasmonates, could explain previously reported positive effects of INCYDE under mild stress conditions. View Full-Text
Keywords: cytokinin; CKX; inhibitor of cytokinin degradation; proteome; Arabidopsis thaliana; stress response attenuation cytokinin; CKX; inhibitor of cytokinin degradation; proteome; Arabidopsis thaliana; stress response attenuation
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MDPI and ACS Style

Berková, V.; Kameniarová, M.; Ondrisková, V.; Berka, M.; Menšíková, S.; Kopecká, R.; Luklová, M.; Novák, J.; Spíchal, L.; Rashotte, A.M.; Brzobohatý, B.; Černý, M. Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome. Plants 2020, 9, 1563. https://doi.org/10.3390/plants9111563

AMA Style

Berková V, Kameniarová M, Ondrisková V, Berka M, Menšíková S, Kopecká R, Luklová M, Novák J, Spíchal L, Rashotte AM, Brzobohatý B, Černý M. Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome. Plants. 2020; 9(11):1563. https://doi.org/10.3390/plants9111563

Chicago/Turabian Style

Berková, Veronika, Michaela Kameniarová, Vladěna Ondrisková, Miroslav Berka, Simona Menšíková, Romana Kopecká, Markéta Luklová, Jan Novák, Lukáš Spíchal, Aaron M. Rashotte, Břetislav Brzobohatý, and Martin Černý. 2020. "Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome" Plants 9, no. 11: 1563. https://doi.org/10.3390/plants9111563

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