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Open AccessArticle

Metabolic Responses to Waterlogging Differ between Roots and Shoots and Reflect Phloem Transport Alteration in Medicago truncatula

1
Seedling Metabolism and Stress, Université d’Angers, Agrocampus Ouest, INRAE, UMR IRHS, SFR QuaSaV, 49071 Beaucouzé, France
2
Research School of Biology, ANU Joint College of Science, Australian National University, Canberra, ACT 2601, Australia
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(10), 1373; https://doi.org/10.3390/plants9101373
Received: 15 September 2020 / Revised: 1 October 2020 / Accepted: 2 October 2020 / Published: 15 October 2020
(This article belongs to the Special Issue Plant Responses to Hypoxia)
Root oxygen deficiency that is induced by flooding (waterlogging) is a common situation in many agricultural areas, causing considerable loss in yield and productivity. Physiological and metabolic acclimation to hypoxia has mostly been studied on roots or whole seedlings under full submergence. The metabolic difference between shoots and roots during waterlogging, and how roots and shoots communicate in such a situation is much less known. In particular, the metabolic acclimation in shoots and how this, in turn, impacts on roots metabolism is not well documented. Here, we monitored changes in the metabolome of roots and shoots of barrel clover (Medicago truncatula), growth, and gas-exchange, and analyzed phloem sap exudate composition. Roots exhibited a typical response to hypoxia, such as γ-aminobutyrate and alanine accumulation, as well as a strong decline in raffinose, sucrose, hexoses, and pentoses. Leaves exhibited a strong increase in starch, sugars, sugar derivatives, and phenolics (tyrosine, tryptophan, phenylalanine, benzoate, ferulate), suggesting an inhibition of sugar export and their alternative utilization by aromatic compounds production via pentose phosphates and phosphoenolpyruvate. Accordingly, there was an enrichment in sugars and a decline in organic acids in phloem sap exudates under waterlogging. Mass-balance calculations further suggest an increased imbalance between loading by shoots and unloading by roots under waterlogging. Taken as a whole, our results are consistent with the inhibition of sugar import by waterlogged roots, leading to an increase in phloem sugar pool, which, in turn, exert negative feedback on sugar metabolism and utilization in shoots. View Full-Text
Keywords: waterlogging; hypoxia; metabolomics; phloem waterlogging; hypoxia; metabolomics; phloem
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Lothier, J.; Diab, H.; Cukier, C.; Limami, A.M.; Tcherkez, G. Metabolic Responses to Waterlogging Differ between Roots and Shoots and Reflect Phloem Transport Alteration in Medicago truncatula. Plants 2020, 9, 1373.

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