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Insertional Mutagenesis Approaches and Their Use in Rice for Functional Genomics

Department of Agri-Biotechnology, National Agri-Food Biotechnology Institute (NABI), Sector-81, SAS Nagar, Mohali 140306, India
Department of Botany, Rajasthan University, Jaipur 302004, India
Authors to whom correspondence should be addressed.
Equal Contributions.
Plants 2019, 8(9), 310;
Received: 12 July 2019 / Revised: 7 August 2019 / Accepted: 7 August 2019 / Published: 29 August 2019
(This article belongs to the Special Issue Advances in Plant Mutagenesis Research)
Insertional mutagenesis is an indispensable tool for engendering a mutant population using exogenous DNA as the mutagen. The advancement in the next-generation sequencing platform has allowed for faster screening and analysis of generated mutated populations. Rice is a major staple crop for more than half of the world’s population; however, the functions of most of the genes in its genome are yet to be analyzed. Various mutant populations represent extremely valuable resources in order to achieve this goal. Here, we have reviewed different insertional mutagenesis approaches that have been used in rice, and have discussed their principles, strengths, and limitations. Comparisons between transfer DNA (T-DNA), transposons, and entrapment tagging approaches have highlighted their utilization in functional genomics studies in rice. We have also summarised different forward and reverse genetics approaches used for screening of insertional mutant populations. Furthermore, we have compiled information from several efforts made using insertional mutagenesis approaches in rice. The information presented here would serve as a database for rice insertional mutagenesis populations. We have also included various examples which illustrate how these populations have been useful for rice functional genomics studies. The information provided here will be very helpful for future functional genomics studies in rice aimed at its genetic improvement. View Full-Text
Keywords: insertional mutagenesis; rice; T-DNA; transposons; functional genomics insertional mutagenesis; rice; T-DNA; transposons; functional genomics
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Ram, H.; Soni, P.; Salvi, P.; Gandass, N.; Sharma, A.; Kaur, A.; Sharma, T.R. Insertional Mutagenesis Approaches and Their Use in Rice for Functional Genomics. Plants 2019, 8, 310.

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