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Open AccessArticle

Proteome Profiling of the Mutagen-Induced Morphological and Yield Macro-Mutant Lines of Nigella sativa L.

1
Department of Botany, Aligarh Muslim University, Aligarh 202002, India
2
Botany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia
3
Mycology and Plant Disease Survey Department, Plant Pathology Research Institute, ARC, Gaza 12511, Egypt
4
Toxicology Laboratory, Department of Zoology, Govt. Raza PG College, Rampur 244901, UP, India
5
Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia
*
Author to whom correspondence should be addressed.
Plants 2019, 8(9), 321; https://doi.org/10.3390/plants8090321
Received: 9 July 2019 / Revised: 23 August 2019 / Accepted: 26 August 2019 / Published: 2 September 2019
(This article belongs to the Special Issue Advances in Plant Mutagenesis Research)
In the present investigation, the leaf proteome profile of the macro-mutant lines of Nigella sativa L. was analyzed to identify the key proteins involved in the expression of traits associated with the morphology, seed yield, and content of thymoquinone. In our earlier study, the macro-mutants were generated with contrasting morphological traits and seed yields through induced mutagenesis, using ethyl methyl sulfonate, gamma rays, and combinations of both. Analysis of the leaf proteome of the control and macro-mutant lines of N. sativa showed that twenty-three proteins were differentially expressed. These differentially expressed proteins were sequenced through mass spectrometry and identified using the MASCOT software. On the basis of their function, these proteins were categorized into several groups. Most proteins were found in the categories of signal transduction (18%) and carbon metabolism (18%). A total of 13% of proteins belonged to the categories of energy and metabolism. Proteins in the categories of secondary plant metabolism, stress defense, cytoskeleton, and protein synthesis were also found. The polycomb group protein (FIE1), transcription factor (PRE1), and geranyl diphosphate synthase were notable proteins, in addition to some proteins of signal transduction and carbon metabolism. Expression patterns of the differentially expressed proteins were also studied at the transcript level by using qRT-PCR. Transcriptomics analysis was consistent with the proteomics data. This study shows the changes that take place at the proteomic level through induced mutagenesis, as well as the involvement of some proteins in the expression traits associated with plant height, seed yield, and the thymoquinone content of N. sativa. The identified proteins might help elucidate the metabolic pathways involved in the expression of traits, including seed yield, and the active compounds of medicinal plants. View Full-Text
Keywords: black cumin; medicinal plant; mutagens; mutant lines; Nigella sativa; proteomics black cumin; medicinal plant; mutagens; mutant lines; Nigella sativa; proteomics
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Asif, A.; K. Ansari, M.Y.; Hashem, A.; Tabassum, B.; Abd_Allah, E.F.; Ahmad, A. Proteome Profiling of the Mutagen-Induced Morphological and Yield Macro-Mutant Lines of Nigella sativa L.. Plants 2019, 8, 321.

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