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Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement

1
National Agri-food Biotechnology Institute (NABI), Mohali, Punjab 140308, India
2
Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India
3
Department of Seed Science and Technology, College of Horticulture, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh 173230, India
4
National Bureau of Plant Genetic Resources, New Delhi 110012, India
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Plants 2019, 8(6), 164; https://doi.org/10.3390/plants8060164
Received: 9 May 2019 / Revised: 27 May 2019 / Accepted: 5 June 2019 / Published: 10 June 2019
(This article belongs to the Special Issue Advances in Plant Mutagenesis Research)
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Abstract

Fast neutron (FN) radiation mediated mutagenesis is a unique approach among the several induced mutagenesis methods being used in plant science in terms of impacted mutations. The FN mutagenesis usually creates deletions from few bases to several million bases (Mb). A library of random deletion generated using FN mutagenesis lines can provide indispensable resources for the reverse genetic approaches. In this review, information from several efforts made using FN mutagenesis has been compiled to understand the type of induced mutations, frequency, and genetic stability. Concerns regarding the utilization of FN mutagenesis technique for a plant with different level of ploidy and genome complexity are discussed. We have highlighted the utility of next-generation sequencing techniques that can be efficiently utilized for the characterization of mutant lines as well as for the mapping of causal mutations. Pros and cons of mapping by mutation (MutMap), mutant chromosome sequencing (MutChromSeq), exon capture, whole genome sequencing, MutRen-Seq, and different tilling approaches that can be used for the detection of FN-induced mutation has also been discussed. Genomic resources developed using the FN mutagenesis have been catalogued wooing to meaningful utilization of the available resources. The information provided here will be helpful for the efficient exploration for the crop improvement programs and for better understanding of genetic regulations. View Full-Text
Keywords: induced mutagenesis; fast neutron irradiation; mutation mapping; deletagene; next-generation sequencing technologies; reverse genetics induced mutagenesis; fast neutron irradiation; mutation mapping; deletagene; next-generation sequencing technologies; reverse genetics
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Kumawat, S.; Rana, N.; Bansal, R.; Vishwakarma, G.; Mehetre, S.T.; Das, B.K.; Kumar, M.; Yadav, S.K.; Sonah, H.; Sharma, T.R.; Deshmukh, R. Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement. Plants 2019, 8, 164.

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