The Copper Amine Oxidase AtCuAOδ Participates in Abscisic Acid-Induced Stomatal Closure in Arabidopsis
AbstractPlant copper amine oxidases (CuAOs) are involved in wound healing, defense against pathogens, methyl-jasmonate-induced protoxylem differentiation, and abscisic acid (ABA)-induced stomatal closure. In the present study, we investigated the role of the Arabidopsis thaliana CuAOδ (AtCuAOδ; At4g12290) in the ABA-mediated stomatal closure by genetic and pharmacological approaches. Obtained data show that AtCuAOδ is up-regulated by ABA and that two Atcuaoδ T-DNA insertional mutants are less responsive to this hormone, showing reduced ABA-mediated stomatal closure and H2O2 accumulation in guard cells as compared to the wild-type (WT) plants. Furthermore, CuAO inhibitors, as well as the hydrogen peroxide (H2O2) scavenger N,N1-dimethylthiourea, reversed most of the ABA-induced stomatal closure in WT plants. Consistently, AtCuAOδ over-expressing transgenic plants display a constitutively increased stomatal closure and increased H2O2 production compared to WT plants. Our data suggest that AtCuAOδ is involved in the H2O2 production related to ABA-induced stomatal closure. View Full-Text
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Fraudentali, I.; Ghuge, S.A.; Carucci, A.; Tavladoraki, P.; Angelini, R.; Cona, A.; Rodrigues-Pousada, R.A. The Copper Amine Oxidase AtCuAOδ Participates in Abscisic Acid-Induced Stomatal Closure in Arabidopsis. Plants 2019, 8, 183.
Fraudentali I, Ghuge SA, Carucci A, Tavladoraki P, Angelini R, Cona A, Rodrigues-Pousada RA. The Copper Amine Oxidase AtCuAOδ Participates in Abscisic Acid-Induced Stomatal Closure in Arabidopsis. Plants. 2019; 8(6):183.Chicago/Turabian Style
Fraudentali, Ilaria; Ghuge, Sandip A.; Carucci, Andrea; Tavladoraki, Paraskevi; Angelini, Riccardo; Cona, Alessandra; Rodrigues-Pousada, Renato A. 2019. "The Copper Amine Oxidase AtCuAOδ Participates in Abscisic Acid-Induced Stomatal Closure in Arabidopsis." Plants 8, no. 6: 183.
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