Next Article in Journal
Metabolic Changes Induced by Silver Ions in Carlina acaulis
Next Article in Special Issue
The Induction and Roles Played by Phi Thickenings in Orchid Roots
Previous Article in Journal
Effect of Hexavalent Chromium [Cr(VI)] on Phytoremediation Potential and Biochemical Response of Hybrid Napier Grass with and without EDTA Application
Previous Article in Special Issue
Arabinogalactan-Proteins from the Liverwort Marchantia polymorpha L., a Member of a Basal Land Plant Lineage, Are Structurally Different to Those of Angiosperms
Open AccessReview

The Suitability of Orthogonal Hosts to Study Plant Cell Wall Biosynthesis

1
Institute for Plant Cell Biology and Biotechnology, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany
2
Independent Junior Research Group–Designer Glycans, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
3
Institute of Synthetic Biology, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany
4
Institute for Synthetic Microbiology, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany
*
Author to whom correspondence should be addressed.
Plants 2019, 8(11), 516; https://doi.org/10.3390/plants8110516
Received: 22 October 2019 / Revised: 8 November 2019 / Accepted: 15 November 2019 / Published: 17 November 2019
(This article belongs to the Special Issue Plant Cell Wall Biology)
Plant cells are surrounded by an extracellular matrix that consists mainly of polysaccharides. Many molecular components involved in plant cell wall polymer synthesis have been identified, but it remains largely unknown how these molecular players function together to define the length and decoration pattern of a polysaccharide. Synthetic biology can be applied to answer questions beyond individual glycosyltransferases by reconstructing entire biosynthetic machineries required to produce a complete wall polysaccharide. Recently, this approach was successful in establishing the production of heteromannan from several plant species in an orthogonal host—a yeast—illuminating the role of an auxiliary protein in the biosynthetic process. In this review we evaluate to what extent a selection of organisms from three kingdoms of life (Bacteria, Fungi and Animalia) might be suitable for the synthesis of plant cell wall polysaccharides. By identifying their key attributes for glycoengineering as well as analyzing the glycosidic linkages of their native polymers, we present a valuable comparison of their key advantages and limitations for the production of different classes of plant polysaccharides. View Full-Text
Keywords: cell walls; polysaccharides; synthetic biology; glycosyltransferases; heterologous expression cell walls; polysaccharides; synthetic biology; glycosyltransferases; heterologous expression
Show Figures

Figure 1

MDPI and ACS Style

Pauly, M.; Gawenda, N.; Wagner, C.; Fischbach, P.; Ramírez, V.; Axmann, I.M.; Voiniciuc, C. The Suitability of Orthogonal Hosts to Study Plant Cell Wall Biosynthesis. Plants 2019, 8, 516.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop