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Open AccessArticle

Identification and Functional Characterization of a Soybean (Glycine max) Thioesterase that Acts on Intermediates of Fatty Acid Biosynthesis

Faculty of Biology and Biotechnology, University of Science, Vietnam National University HCMC, Ho Chi Minh 700000, Vietnam
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Plants 2019, 8(10), 397; https://doi.org/10.3390/plants8100397
Received: 26 August 2019 / Revised: 21 September 2019 / Accepted: 2 October 2019 / Published: 8 October 2019
(This article belongs to the Section Plant Physiology and Metabolism)
(1) Background: Plants possess many acyl-acyl carrier protein (acyl-ACP) thioesterases (TEs) with unique specificity. One such TE is methylketone synthase 2 (MKS2), an enzyme with a single-hotdog-fold structure found in several tomato species that hydrolyzes 3-ketoacyl-ACPs to give free 3-ketoacids. (2) Methods: In this study, we identified and characterized a tomato MKS2 homolog gene, namely, GmMKS2, in the genome of soybean (Glycine max). (3) Results: GmMKS2 underwent alternative splicing to produce three alternative transcripts, but only one encodes a protein with thioesterase activity when recombinantly expressed in Escherichia coli. Heterologous expression of the main transcript of GmMKS2, GmMKS2-X2, in E. coli generated various types of fatty acids, including 3-ketoacids—with 3-ketotetradecenoic acid (14:1) being the most abundant—cis-Δ5-dodecanoic acid, and 3-hydroxyacids, suggesting that GmMKS2 acts as an acyl-ACP thioesterase. In plants, the GmMKS2-X2 transcript level was found to be higher in the roots compared to other examined organs. In silico analysis revealed that there is a substantial enrichment of putative cis-regulatory elements related to disease-resistance responses and abiotic stress responses in the promoter of this gene. (4) Conclusions: GmMKS2 showed broad substrate specificities toward a wide range of acyl-ACPs that varied in terms of chain length, oxidation state, and saturation degree. Our results suggest that GmMKS2 might have a stress-related physiological function in G. max. View Full-Text
Keywords: 2-methylketones; 3-hydroxyacids; 3-ketoacids; acyl-ACP thioesterase; Glycine max; methylketone synthase 2 (MKS2) 2-methylketones; 3-hydroxyacids; 3-ketoacids; acyl-ACP thioesterase; Glycine max; methylketone synthase 2 (MKS2)
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Tran, H.T.D.; Le, N.T.; Khuat, V.L.U.; Nguyen, T.T.H. Identification and Functional Characterization of a Soybean (Glycine max) Thioesterase that Acts on Intermediates of Fatty Acid Biosynthesis. Plants 2019, 8, 397.

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