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Volume 11
Issue 2
10.3390/plants11020171
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Article

Effect of Calmodulin-like Gene (CML) Overexpression on Stilbene Biosynthesis in Cell Cultures of Vitis amurensis Rupr.

by
Olga A. Aleynova
1,
Andrey R. Suprun
1,
Alexey A. Ananev
1,2,
Nikolay N. Nityagovsky
1,
Zlata V. Ogneva
1,
Alexandra S. Dubrovina
1 and
Konstantin V. Kiselev
1,*
1
Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, FEB RAS, 690022 Vladivostok, Russia
2
Department of Biochemistry and Biotechnology, Institute of the World Ocean, Far Eastern Federal University, 690090 Vladivostok, Russia
*
Author to whom correspondence should be addressed.
Plants 2022, 11(2), 171; https://doi.org/10.3390/plants11020171
Submission received: 28 December 2021 / Accepted: 5 January 2022 / Published: 10 January 2022
(This article belongs to the Special Issue Calcium Signaling in Plants: Roles in Stress Tolerance and Development)
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Abstract

Stilbenes are plant phenolics known to rapidly accumulate in grapevine and other plants in response to injury or pathogen attack and to exhibit a great variety of healing beneficial effects. It has previously been shown that several calmodulin-like protein (CML) genes were highly up-regulated in cell cultures of wild-growing grapevine Vitis amurensis Rupr. in response to stilbene-modulating conditions, such as stress hormones, UV-C, and stilbene precursors. Both CML functions and stilbene biosynthesis regulation are still poorly understood. In this study, we investigated the effect of overexpression of five VaCML genes on stilbene and biomass accumulation in the transformed cell cultures of V. amurensis. We obtained 16 transgenic cell lines transformed with the VaCML52, VaCML65, VaCML86, VaCML93, and VaCML95 genes (3–4 independent lines per gene) under the control of the double CaMV 35S promoter. HPLC-MS analysis showed that overexpression of the VaCML65 led to a considerable and consistent increase in the content of stilbenes of 3.8–23.7 times in all transformed lines in comparison with the control calli, while biomass accumulation was not affected. Transformation of the V. amurensis cells with other analyzed VaCML genes did not lead to a consistent and considerable effect on stilbene biosynthesis in the cell lines. The results indicate that the VaCML65 gene is implicated in the signaling pathway regulating stilbene biosynthesis as a strong positive regulator and can be useful in viticulture and winemaking for obtaining grape cultivars with a high content of stilbenes and stress resistance.
Keywords: CML; calcium; stilbenes; transgenic cells; plant cell cultures CML; calcium; stilbenes; transgenic cells; plant cell cultures

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MDPI and ACS Style

Aleynova, O.A.; Suprun, A.R.; Ananev, A.A.; Nityagovsky, N.N.; Ogneva, Z.V.; Dubrovina, A.S.; Kiselev, K.V. Effect of Calmodulin-like Gene (CML) Overexpression on Stilbene Biosynthesis in Cell Cultures of Vitis amurensis Rupr. Plants 2022, 11, 171. https://doi.org/10.3390/plants11020171

AMA Style

Aleynova OA, Suprun AR, Ananev AA, Nityagovsky NN, Ogneva ZV, Dubrovina AS, Kiselev KV. Effect of Calmodulin-like Gene (CML) Overexpression on Stilbene Biosynthesis in Cell Cultures of Vitis amurensis Rupr. Plants. 2022; 11(2):171. https://doi.org/10.3390/plants11020171

Chicago/Turabian Style

Aleynova, Olga A., Andrey R. Suprun, Alexey A. Ananev, Nikolay N. Nityagovsky, Zlata V. Ogneva, Alexandra S. Dubrovina, and Konstantin V. Kiselev. 2022. "Effect of Calmodulin-like Gene (CML) Overexpression on Stilbene Biosynthesis in Cell Cultures of Vitis amurensis Rupr." Plants 11, no. 2: 171. https://doi.org/10.3390/plants11020171

APA Style

Aleynova, O. A., Suprun, A. R., Ananev, A. A., Nityagovsky, N. N., Ogneva, Z. V., Dubrovina, A. S., & Kiselev, K. V. (2022). Effect of Calmodulin-like Gene (CML) Overexpression on Stilbene Biosynthesis in Cell Cultures of Vitis amurensis Rupr. Plants, 11(2), 171. https://doi.org/10.3390/plants11020171

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