Moderate Salinity Stress Affects Expression of Main Sugar Metabolism and Transport Genes and Soluble Carbohydrate Content in Ripe Fig Fruits (Ficus carica L. cv. Dottato)
Abstract
:1. Introduction
2. Results
2.1. Salt-Induced Expression of Genes Involved in Soluble Carbohydrate Metabolism and Transport
2.2. Changes in Main Soluble Carbohydrates Contents in Response to Salinity
3. Discussion
4. Materials and Methods
4.1. Plant Material and Salt Treatment
4.2. Nucleic Acid Isolation and Analysis of Gene Expression
4.3. Quantitative 1H Nuclear Magnetic Resonance (NMR) for the Determination of Free Soluble Carbohydrates
4.4. Experimental Design and Statistical Analysis of Data
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Soluble Carbohydrates | Control | 100 mM NaCl-Stressed | p-Value |
---|---|---|---|
D-Fructose | 242.68 ± 7.73 | 235.87 ± 3.64 | 0.24 |
D-Glucose | 240.92 ± 15.71 | 219.30 ± 5.29 | 0.09 |
Sucrose | 27.9 ± 0.3 | 34.31 ± 3.51 | 0.03 |
D-Sorbitol | 0.99 ± 0.23 | 1.48 ± 0.15 | 0.04 |
Total | 511.68 ± 23.04 | 490.97 ± 2.14 | 0.18 |
Ratios | |||
Glucose/Fructose | 99.22 ± 3.76 | 92.99 ± 3.04 | 0.09 |
Sucrose/Fructose | 11.5 ± 0.25 | 14.55 ± 1.55 | 0.03 |
Sucrose/Glucose | 11.61 ± 0.64 | 15.68 ± 1.95 | 0.03 |
Sucrose/Glucose + Fructose | 5.78 ± 0.22 | 7.55 ± 0.87 | 0.03 |
Sorbitol/Sucrose | 3.55 ± 0.82 | 4.35 ± 0.69 | 0.26 |
Sorbitol/Glucose | 0.42 ± 0.12 | 0.68 ± 0.08 | 0.03 |
Sorbitol/Fructose | 0.41 ± 0.10 | 0.63 ± 0.06 | 0.03 |
Sorbitol/Glucose + Fructose | 0.21 ± 0.05 | 0.33 ± 0.03 | 0.03 |
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Mascellani, A.; Natali, L.; Cavallini, A.; Mascagni, F.; Caruso, G.; Gucci, R.; Havlik, J.; Bernardi, R. Moderate Salinity Stress Affects Expression of Main Sugar Metabolism and Transport Genes and Soluble Carbohydrate Content in Ripe Fig Fruits (Ficus carica L. cv. Dottato). Plants 2021, 10, 1861. https://doi.org/10.3390/plants10091861
Mascellani A, Natali L, Cavallini A, Mascagni F, Caruso G, Gucci R, Havlik J, Bernardi R. Moderate Salinity Stress Affects Expression of Main Sugar Metabolism and Transport Genes and Soluble Carbohydrate Content in Ripe Fig Fruits (Ficus carica L. cv. Dottato). Plants. 2021; 10(9):1861. https://doi.org/10.3390/plants10091861
Chicago/Turabian StyleMascellani, Anna, Lucia Natali, Andrea Cavallini, Flavia Mascagni, Giovanni Caruso, Riccardo Gucci, Jaroslav Havlik, and Rodolfo Bernardi. 2021. "Moderate Salinity Stress Affects Expression of Main Sugar Metabolism and Transport Genes and Soluble Carbohydrate Content in Ripe Fig Fruits (Ficus carica L. cv. Dottato)" Plants 10, no. 9: 1861. https://doi.org/10.3390/plants10091861