Next Article in Journal
‘Pinore’: The New Red Wine Variety Cross-Bred between ‘Pinot Noir’ and ‘Regent’ Vines
Next Article in Special Issue
MdMADS6 Recruits Histone Deacetylase MdHDA19 to Repress the Expression of the Carotenoid Synthesis-Related Gene MdCCD1 during Fruit Ripening
Previous Article in Journal
Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses
Previous Article in Special Issue
The RNA Directed DNA Methylation (RdDM) Pathway Regulates Anthocyanin Biosynthesis in Crabapple (Malus cv. spp.) Leaves by Methylating the McCOP1 Promoter
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Plants
Volume 10
Issue 12
10.3390/plants10122665
plants-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy

by
Julio Garighan
1,†,
Etienne Dvorak
1,†,
Joan Estevan
1,
Karine Loridon
1,
Bruno Huettel
2,
Gautier Sarah
1,
Isabelle Farrera
1,
Julie Leclercq
1,3,
Priscila Grynberg
4,
Roberto Coiti Togawa
4,
Marcos Mota do Carmo Costa
4,
Evelyne Costes
1 and
Fernando Andrés
1,*
1
UMR AGAP Institute, Institut Agro, CIRAD, INRAE, University of Montpellier, F-34398 Montpellier, France
2
Genome Centre, Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany
3
UMR AGAP Institute, CIRAD, F-34398 Montpellier, France
4
Bioinformatica Laboratory, Embrapa Recursos Genéticos e Biotecnologia—Cenargen, Brasilia 02372, Brazil
*
Author to whom correspondence should be addressed.
†
Equal contribution.
Plants 2021, 10(12), 2665; https://doi.org/10.3390/plants10122665
Submission received: 29 October 2021 / Revised: 1 December 2021 / Accepted: 2 December 2021 / Published: 3 December 2021
(This article belongs to the Special Issue Malus Biology)
Browse Figures
Versions Notes

Abstract

Winter dormancy is an adaptative mechanism that temperate and boreal trees have developed to protect their meristems against low temperatures. In apple trees (Malus domestica), cold temperatures induce bud dormancy at the end of summer/beginning of the fall. Apple buds stay dormant during winter until they are exposed to a period of cold, after which they can resume growth (budbreak) and initiate flowering in response to warmer temperatures in spring. It is well-known that small RNAs modulate temperature responses in many plant species, but however, how small RNAs are involved in genetic networks of temperature-mediated dormancy control in fruit tree species remains unclear. Here, we have made use of a recently developed ARGONAUTE (AGO)-purification technique to isolate small RNAs from apple buds. A small RNA-seq experiment resulted in the identification of 17 micro RNAs (miRNAs) that change their pattern of expression in apple buds during dormancy. Furthermore, the functional analysis of their predicted target genes suggests a main role of the 17 miRNAs in phenylpropanoid biosynthesis, gene regulation, plant development and growth, and response to stimulus. Finally, we studied the conservation of the Arabidopsis thaliana regulatory miR159-MYB module in apple in the context of the plant hormone abscisic acid homeostasis.
Keywords: dormancy; apple tree; small RNAs; miR159 dormancy; apple tree; small RNAs; miR159

Share and Cite

MDPI and ACS Style

Garighan, J.; Dvorak, E.; Estevan, J.; Loridon, K.; Huettel, B.; Sarah, G.; Farrera, I.; Leclercq, J.; Grynberg, P.; Coiti Togawa, R.; et al. The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy. Plants 2021, 10, 2665. https://doi.org/10.3390/plants10122665

AMA Style

Garighan J, Dvorak E, Estevan J, Loridon K, Huettel B, Sarah G, Farrera I, Leclercq J, Grynberg P, Coiti Togawa R, et al. The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy. Plants. 2021; 10(12):2665. https://doi.org/10.3390/plants10122665

Chicago/Turabian Style

Garighan, Julio, Etienne Dvorak, Joan Estevan, Karine Loridon, Bruno Huettel, Gautier Sarah, Isabelle Farrera, Julie Leclercq, Priscila Grynberg, Roberto Coiti Togawa, and et al. 2021. "The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy" Plants 10, no. 12: 2665. https://doi.org/10.3390/plants10122665

APA Style

Garighan, J., Dvorak, E., Estevan, J., Loridon, K., Huettel, B., Sarah, G., Farrera, I., Leclercq, J., Grynberg, P., Coiti Togawa, R., Mota do Carmo Costa, M., Costes, E., & Andrés, F. (2021). The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy. Plants, 10(12), 2665. https://doi.org/10.3390/plants10122665

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop