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Open AccessArticle

Overexpression of Banana ATG8f Modulates Drought Stress Resistance in Arabidopsis

by Bing Li 1,†, Guoyin Liu 1,2,†, Yuqi Wang 2, Yunxie Wei 1,* and Haitao Shi 1,*
1
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou 570228, China
2
College of Forestry, Hainan University, Haikou 570228, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomolecules 2019, 9(12), 814; https://doi.org/10.3390/biom9120814
Received: 12 November 2019 / Revised: 28 November 2019 / Accepted: 28 November 2019 / Published: 2 December 2019
(This article belongs to the Special Issue Redox Regulation of Protein Functioning)
Autophagy is essential for plant growth, development, and stress resistance. However, the involvement of banana autophagy-related genes in drought stress response and the underlying mechanism remain elusive. In this study, we found that the transcripts of 10 banana ATG8s responded to drought stress in different ways, and MaATG8f with the highest transcript in response to drought stress among them was chosen for functional analysis. Overexpression of MaATG8f improved drought stress resistance in  Arabidopsis,with lower malonaldehyde level and higher level of assimilation rate. On the one hand, overexpression of MaATG8f activated the activities of superoxide dismutase, catalase, and peroxidase under drought stress conditions, so as to regulate reactive oxygen species accumulation. On the other hand, MaATG8f-overexpressing lines exhibited higher endogenous abscisic acid (ABA) level and more sensitivity to abscisic acid. Notably, the autophagosomes as visualized by CaMV35S::GFP–MaATG8f was activated after ABA treatment. Taken together, overexpression of MaATG8f positively regulated plant drought stress resistance through modulating reactive oxygen species metabolism, abscisic acid biosynthesis, and autophagic activity.
Keywords: autophagy; MaATG8f; drought; abscisic acid; reactive oxygen species; banana autophagy; MaATG8f; drought; abscisic acid; reactive oxygen species; banana
MDPI and ACS Style

Li, B.; Liu, G.; Wang, Y.; Wei, Y.; Shi, H. Overexpression of Banana ATG8f Modulates Drought Stress Resistance in Arabidopsis. Biomolecules 2019, 9, 814.

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