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Article

Tomato Root Growth Inhibition by Salinity and Cadmium is Mediated by S-Nitrosative Modifications of ROS Metabolic Enzymes Controlled by S-Nitrosoglutathione Reductase

1
Department of Biochemistry, Faculty of Science, Palacký University, CZ-783 71 Olomouc, Czech Republic
2
Present address: Department of Immunology, Faculty of Medicine and Dentistry, Palacký University, CZ-77900 Olomouc, Czech Republic
3
Present address: Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, 760 01 Zlín, Czech Republic
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(9), 393; https://doi.org/10.3390/biom9090393
Received: 28 June 2019 / Revised: 14 August 2019 / Accepted: 19 August 2019 / Published: 21 August 2019
(This article belongs to the Special Issue Redox Regulation of Protein Functioning)
S-nitrosoglutathione reductase (GSNOR) exerts crucial roles in the homeostasis of nitric oxide (NO) and reactive nitrogen species (RNS) in plant cells through indirect control of S-nitrosation, an important protein post-translational modification in signaling pathways of NO. Using cultivated and wild tomato species, we studied GSNOR function in interactions of key enzymes of reactive oxygen species (ROS) metabolism with RNS mediated by protein S-nitrosation during tomato root growth and responses to salinity and cadmium. Application of a GSNOR inhibitor N6022 increased both NO and S-nitrosothiol levels and stimulated root growth in both genotypes. Moreover, N6022 treatment, as well as S-nitrosoglutathione (GSNO) application, caused intensive S-nitrosation of important enzymes of ROS metabolism, NADPH oxidase (NADPHox) and ascorbate peroxidase (APX). Under abiotic stress, activities of APX and NADPHox were modulated by S-nitrosation. Increased production of H2O2 and subsequent oxidative stress were observed in wild Solanum habrochaites, together with increased GSNOR activity and reduced S-nitrosothiols. An opposite effect occurred in cultivated S. lycopersicum, where reduced GSNOR activity and intensive S-nitrosation resulted in reduced ROS levels by abiotic stress. These data suggest stress-triggered disruption of ROS homeostasis, mediated by modulation of RNS and S-nitrosation of NADPHox and APX, underlies tomato root growth inhibition by salinity and cadmium stress. View Full-Text
Keywords: abiotic stress; cadmium; nitric oxide; reactive oxygen species; root growth; S-nitrosation; S-nitrosoglutathione reductase; salinity; Solanum habrochaites; Solanum lycopersicum abiotic stress; cadmium; nitric oxide; reactive oxygen species; root growth; S-nitrosation; S-nitrosoglutathione reductase; salinity; Solanum habrochaites; Solanum lycopersicum
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MDPI and ACS Style

Jedelská, T.; Šmotková Kraiczová, V.; Berčíková, L.; Činčalová, L.; Luhová, L.; Petřivalský, M. Tomato Root Growth Inhibition by Salinity and Cadmium is Mediated by S-Nitrosative Modifications of ROS Metabolic Enzymes Controlled by S-Nitrosoglutathione Reductase. Biomolecules 2019, 9, 393. https://doi.org/10.3390/biom9090393

AMA Style

Jedelská T, Šmotková Kraiczová V, Berčíková L, Činčalová L, Luhová L, Petřivalský M. Tomato Root Growth Inhibition by Salinity and Cadmium is Mediated by S-Nitrosative Modifications of ROS Metabolic Enzymes Controlled by S-Nitrosoglutathione Reductase. Biomolecules. 2019; 9(9):393. https://doi.org/10.3390/biom9090393

Chicago/Turabian Style

Jedelská, Tereza, Veronika Šmotková Kraiczová, Lucie Berčíková, Lucie Činčalová, Lenka Luhová, and Marek Petřivalský. 2019. "Tomato Root Growth Inhibition by Salinity and Cadmium is Mediated by S-Nitrosative Modifications of ROS Metabolic Enzymes Controlled by S-Nitrosoglutathione Reductase" Biomolecules 9, no. 9: 393. https://doi.org/10.3390/biom9090393

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