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Open AccessArticle

Anthocyanins from Hibiscus syriacus L. Inhibit Melanogenesis by Activating the ERK Signaling Pathway

1
Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea
2
Forest Biomaterials Research Center, National Institute of Forest Science, Jinju 52817, Korea
3
Forest Tree Improvement Division, Bioresources Research Center, National Institute of Forest Science, Suwon 1663, Korea
4
Department of Molecular Biology, Ariel University, Science Park, Ariel 40700, Israel
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Department of Biochemistry, College of Oriental Medicine, Dong-Eui University, Busan 47227, Korea
6
Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Korea
7
Department of Research Center, Dong Nam Institute of Radiological and Medical Sciences, Busan 46033, Korea
*
Authors to whom correspondence should be addressed.
Biomolecules 2019, 9(11), 645; https://doi.org/10.3390/biom9110645
Received: 27 September 2019 / Revised: 21 October 2019 / Accepted: 22 October 2019 / Published: 24 October 2019
Hibiscus syriacus L. exhibited promising potential as a new source of food and colorants containing various anthocyanins. However, the function of anthocyanins from H. syriacus L. has not been investigated. In the current study, we evaluated whether anthocyanins from the H. syriacus L. varieties Pulsae and Paektanshim (PS and PTS) inhibit melanin biogenesis. B16F10 cells and zebrafish larvae were exposed to PS and PTS in the presence or absence of α-melanocyte-stimulating hormone (α-MSH), and melanin contents accompanied by its regulating genes and proteins were analyzed. PS and PTS moderately downregulated mushroom tyrosinase activity in vitro, but significantly decreased extracellular and intracellular melanin production in B16F10 cells, and inhibited α-MSH-induced expression of microphthalmia-associated transcription factor (MITF) and tyrosinase. PS and PTS also attenuated pigmentation in α-MSH-stimulated zebrafish larvae. Furthermore, PS and PTS activated the phosphorylation of extracellular signal-regulated kinase (ERK), whereas PD98059, a specific ERK inhibitor, completely reversed PS- and PTS-mediated anti-melanogenic activity in B16F10 cells and zebrafish larvae, which indicates that PS- and PTS-mediated anti-melanogenic activity is due to ERK activation. Moreover, chromatography data showed that PS and PTS possessed 17 identical anthocyanins as a negative regulator of ERK. These findings suggested that anthocyanins from PS and PTS inhibited melanogenesis in vitro and in vivo by activating the ERK signaling pathway. View Full-Text
Keywords: Hibiscus syriacus L.; anthocyanin; melanin; tyrosinase; ERK Hibiscus syriacus L.; anthocyanin; melanin; tyrosinase; ERK
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Karunarathne, W.A.H.M.; Molagoda, I.M.N.; Park, S.R.; Kim, J.W.; Lee, O.-K.; Kwon, H.Y.; Oren, M.; Choi, Y.H.; Ryu, H.W.; Oh, S.-R.; Jo, W.S.; Lee, K.T.; Kim, G.-Y. Anthocyanins from Hibiscus syriacus L. Inhibit Melanogenesis by Activating the ERK Signaling Pathway. Biomolecules 2019, 9, 645.

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