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Suppression of Sclerotinia sclerotiorum by the Induction of Systemic Resistance and Regulation of Antioxidant Pathways in Tomato Using Fengycin Produced by Bacillus amyloliquefaciens FZB42

1
College of Plant Protection, Department of Plant Pathology, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Disease and Pest Insects, Ministry of Agriculture, Nanjing 210095, China
2
Department of Plant Pathology, University of Agriculture, Faisalabad, P.O. Box 38040, Pakistan
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(10), 613; https://doi.org/10.3390/biom9100613
Received: 27 September 2019 / Revised: 10 October 2019 / Accepted: 11 October 2019 / Published: 16 October 2019
Lipopeptides from Bacillus species exhibit promising biological control activity against plant pathogens. This study aimed to explore the potential of purified fengycin to induce systemic resistance in tomato against Sclerotinia sclerotiorum. Bacillus amyloliquefaciens FZB42, its mutant AK1S, and their corresponding metabolites showed in vitro inhibition of S. sclerotiorum mycelium. Fengycin derived from an AK1S mutant was purified and identified through HPLC and MALDI-TOF-MS, respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed structural deformities in the fungal mycelium. Moreover, fengycin induced the accumulation of reactive oxygen species (ROS) in S. sclerotiorum mycelium and downregulated the expression of ROS-scavenging genes viz., superoxide dismutase (SsSOD1), peroxidase (SsPO), and catalase (SsCAT1) compared to the untreated control. Furthermore, the lesion size was dramatically reduced in fengycin-treated tomato plants compared to plants infected with S. sclerotiorum only in a greenhouse experiment. Additionally, the transcriptional regulation of defense-related genes GST, SOD, PAL, HMGR, and MPK3 showed the highest upsurge in expression at 48 h post-inoculation (hpi). However, their expression was subsequently decreased at 96 hpi in fengycin + S. sclerotiorum treatment compared to the plants treated with fengycin only. Conversely, the expression of PPO increased in a linear manner up to 96 hpi. View Full-Text
Keywords: lipopeptide; MALDI-TOF-MS; HPLC; reactive oxygen species; induced systemic resistance; defense-related genes lipopeptide; MALDI-TOF-MS; HPLC; reactive oxygen species; induced systemic resistance; defense-related genes
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MDPI and ACS Style

Farzand, A.; Moosa, A.; Zubair, M.; Khan, A.R.; Massawe, V.C.; Tahir, H.A.S.; Sheikh, T.M.M.; Ayaz, M.; Gao, X. Suppression of Sclerotinia sclerotiorum by the Induction of Systemic Resistance and Regulation of Antioxidant Pathways in Tomato Using Fengycin Produced by Bacillus amyloliquefaciens FZB42. Biomolecules 2019, 9, 613. https://doi.org/10.3390/biom9100613

AMA Style

Farzand A, Moosa A, Zubair M, Khan AR, Massawe VC, Tahir HAS, Sheikh TMM, Ayaz M, Gao X. Suppression of Sclerotinia sclerotiorum by the Induction of Systemic Resistance and Regulation of Antioxidant Pathways in Tomato Using Fengycin Produced by Bacillus amyloliquefaciens FZB42. Biomolecules. 2019; 9(10):613. https://doi.org/10.3390/biom9100613

Chicago/Turabian Style

Farzand, Ayaz; Moosa, Anam; Zubair, Muhammad; Khan, Abdur R.; Massawe, Venance C.; Tahir, Hafiz A.S.; Sheikh, Taha M.M.; Ayaz, Muhammad; Gao, Xuewen. 2019. "Suppression of Sclerotinia sclerotiorum by the Induction of Systemic Resistance and Regulation of Antioxidant Pathways in Tomato Using Fengycin Produced by Bacillus amyloliquefaciens FZB42" Biomolecules 9, no. 10: 613. https://doi.org/10.3390/biom9100613

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