Studies on the Interaction of Alyteserin 1c Peptide and Its Cationic Analogue with Model Membranes Imitating Mammalian and Bacterial Membranes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Peptide Synthesis and Purification
2.3. Helical Wheel Projection of the Peptides
2.4. Model Membranes Preparation
2.5. Differential Scanning Calorimetry (DSC)
2.6. Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR)
2.7. Molecular Modelling and Molecular Dynamics Simulation of the Complex Peptide-Membrane
3. Results and Discussion
3.1. Peptide Sequence
3.2. Thermotropic Behavior of Model Membranes
3.3. Peptide +2 and +5 Secondary Structure before and after Interacting with Membrane Models
3.4. Molecular Dynamics Analysis of the Peptide-Membrane Complex
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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MLV | Peptide–Lipid Molar Ratio | Pre-Transition Temperature (°C) | Tm (°C) | ΔH (J·g−1) |
---|---|---|---|---|
DMPC | 0:1 | 12.9 | 22.9 | 1.47 |
DMPC-Peptide +2 | 1:100 | 21.2 | 0.63 | |
1:50 | 21.5 | 0.62 | ||
1:25 | 21.2 | 0.62 | ||
DMPC-Peptide +5 | 1:100 | 22.7 | 0.8 | |
1:50 | 22.2 | 0.63 | ||
1:25 | 20.5 | 0.18 | ||
DMPC/DMPG (3:1) | 0:1 | 12 | 22.9 | 1.6 |
DMPC/DMPG (3:1)-Peptide +2 | 1:100 | 22.9 | 0.54 | |
1:50 | 22.8 | 0.38 | ||
1:25 | 21.3 | 0.30 | ||
DMPC/DMPG (3:1)-Peptide +5 | 1:100 | 22.9 | 0.64 | |
1:50 | 23 | 0.45 | ||
1:25 | 22.6 | 0.44 |
Peptide | α-Helix (%) | β-Sheet (%) | Turns (%) | Unordered (%) | Aggregation (%) | C=O Band Area/Total Area (%) |
---|---|---|---|---|---|---|
+2 | 66 | 34 | ||||
+2 + POPC | 64 | 36 | 22 | |||
+2 + POPC/POPG | 51 | 49 | 29 | |||
+5 | 48 | 52 | ||||
+5 + POPC | 72 | 28 | 14 | |||
+5 + POPC/POPG | 81 | 19 | 16 |
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Aragón-Muriel, A.; Ausili, A.; Sánchez, K.; Rojas A., O.E.; Londoño Mosquera, J.; Polo-Cerón, D.; Oñate-Garzón, J. Studies on the Interaction of Alyteserin 1c Peptide and Its Cationic Analogue with Model Membranes Imitating Mammalian and Bacterial Membranes. Biomolecules 2019, 9, 527. https://doi.org/10.3390/biom9100527
Aragón-Muriel A, Ausili A, Sánchez K, Rojas A. OE, Londoño Mosquera J, Polo-Cerón D, Oñate-Garzón J. Studies on the Interaction of Alyteserin 1c Peptide and Its Cationic Analogue with Model Membranes Imitating Mammalian and Bacterial Membranes. Biomolecules. 2019; 9(10):527. https://doi.org/10.3390/biom9100527
Chicago/Turabian StyleAragón-Muriel, Alberto, Alessio Ausili, Kevin Sánchez, Oscar E. Rojas A., Juan Londoño Mosquera, Dorian Polo-Cerón, and Jose Oñate-Garzón. 2019. "Studies on the Interaction of Alyteserin 1c Peptide and Its Cationic Analogue with Model Membranes Imitating Mammalian and Bacterial Membranes" Biomolecules 9, no. 10: 527. https://doi.org/10.3390/biom9100527