Total Fatty Acid Analysis of Human Blood Samples in One Minute by High-Resolution Mass Spectrometry
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Standards
2.2. Human Plasma Samples
2.3. Sample Preparation
2.4. Mass Spectrometric Analysis
2.5. Lipid Identification and Quantification
2.6. FAME Analysis
3. Results and Discussion
3.1. Total Fatty Acyl Analysis by Direct Infusion FTMS Analysis
3.2. Hydrolysis with H218O Improves Signal-to-Background
3.3. Instrument Response Is Independent of Fatty Acyl Chain Length and Double Bond Number
3.4. Dynamic Quantification Range of Total Fatty Acyl Analysis by FTMS
3.5. Accurate Fatty Acyl Quantification by High Resolution FTMS Analysis
3.6. Total Fatty Acyl Analysis by FTMS Is Precise and Applicable for Routine Analysis
3.7. Lipotyping of Human Serum Samples by Total Fatty Acyl Analysis
3.8. Quantitative Analysis of Stable Isotope-Labeled Fatty Acyls in Human Plasma
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Intra-Day | Inter-Day | ||||||||
---|---|---|---|---|---|---|---|---|---|
Day 1 (n = 5) | Day 2 (n = 5) | Day 3 (n = 5) | (n= 3 × 5) | ||||||
Analyte | Insulin | µM ± SD | CV (%) | µM ± SD | CV (%) | µM ± SD | CV (%) | µM ± SD | CV (%) |
FA 16:0 | Normal | 1852 ± 103 | 5.5 | 2085 ± 179 | 8.6 | 2028 ± 105 | 5.2 | 1988 ± 161 | 8.1 |
High | 4239 ± 294 | 6.9 | 3798 ± 288 | 7.6 | 4373 ± 218 | 5.0 | 4137 ± 356 | 8.6 | |
FA 18:1 | Normal | 2898 ± 54 | 1.9 | 3132 ± 156 | 5.0 | 3130 ± 183 | 5.8 | 3053 ± 174 | 5.7 |
High | 10859 ± 534 | 4.9 | 9618 ± 279 | 2.9 | 11114 ± 508 | 4.6 | 10531 ± 797 | 7.6 | |
FA 18:2 | Normal | 3404 ± 133 | 3.9 | 3734 ± 192 | 5.1 | 3765 ± 162 | 4.3 | 3634 ± 227 | 6.2 |
High | 3442 ± 275 | 8.0 | * 3031 ± 102 | 3.4 | 3640 ± 412 | 11.3 | 3396 ± 378 | 11.1 | |
FA 20:4 | Normal | 920 ± 24 | 2.6 | 948 ± 26 | 2.8 | 998 ± 80 | 8.0 | 955 ± 58 | 6.0 |
High | 1728 ± 95 | 5.5 | 1504 ± 56 | 3.7 | 1750 ± 111 | 6.4 | 1661 ± 142 | 8.6 | |
FA 20:5 | Normal | 108 ± 3.6 | 3.3 | 115 ± 3.1 | 2.7 | 121 ± 10 | 8.6 | 115 ± 8.2 | 7.1 |
High | 188 ± 8.9 | 4.7 | 165 ± 6.5 | 3.9 | 194 ± 14 | 7.0 | 182 ± 16 | 8.8 | |
FA 22:5 | Normal | 73 ± 3.0 | 4.1 | 76 ± 1.8 | 2.4 | 82 ± 5.9 | 7.2 | 77 ± 5.3 | 6.8 |
High | 146 ± 8.5 | 5.8 | 123 ± 4.4 | 3.6 | 147 ± 9.9 | 6.7 | 139 ± 13 | 9.6 | |
FA 22:6 | Normal | 197 ± 5.2 | 2.7 | 205 ± 5.3 | 2.6 | 216 ± 16 | 7.4 | 206 ± 12 | 6.0 |
High | 163 ± 8.5 | 5.2 | 141 ± 6.4 | 4.5 | 167 ± 14 | 8.6 | 157 ± 15 | 9.7 |
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Gallego, S.F.; Hermansson, M.; Liebisch, G.; Hodson, L.; Ejsing, C.S. Total Fatty Acid Analysis of Human Blood Samples in One Minute by High-Resolution Mass Spectrometry. Biomolecules 2019, 9, 7. https://doi.org/10.3390/biom9010007
Gallego SF, Hermansson M, Liebisch G, Hodson L, Ejsing CS. Total Fatty Acid Analysis of Human Blood Samples in One Minute by High-Resolution Mass Spectrometry. Biomolecules. 2019; 9(1):7. https://doi.org/10.3390/biom9010007
Chicago/Turabian StyleGallego, Sandra F., Martin Hermansson, Gerhard Liebisch, Leanne Hodson, and Christer S. Ejsing. 2019. "Total Fatty Acid Analysis of Human Blood Samples in One Minute by High-Resolution Mass Spectrometry" Biomolecules 9, no. 1: 7. https://doi.org/10.3390/biom9010007