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Biomolecules 2018, 8(4), 171; https://doi.org/10.3390/biom8040171

Anticancer Activity of Polysaccharides Produced from Glycerol and Crude Glycerol by an Endophytic Fungus Chaetomium globosum CGMCC 6882 on Human Lung Cancer A549 Cells

1
College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China
2
The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
3
Department of Biochemistry and Molecular Medicine, UC Davis Comprehensive Cancer Center, University of California Davis, Sacramento, CA 95817, USA
*
Authors to whom correspondence should be addressed.
Received: 2 November 2018 / Revised: 4 December 2018 / Accepted: 6 December 2018 / Published: 11 December 2018
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Abstract

Two polysaccharides were produced by Chaetomium globosum CGMCC 6882 from glycerol (GCP-1) and crude glycerol (GCP-2). Chemical characteristics results showed GCP-1 and GCP-2 were similar polysaccharides, but the molecular weights of GCP-1 and GCP-2 were 5.340 × 104 Da and 3.105 × 104 Da, respectively. Viabilities of A549 cells after treatment with GCP-1 and GCP-2 were 49% and 39% compared to the control group. Meanwhile, flow cytometry results indicated that GCP-1 and GCP-2 could induce 17.79% and 24.28% of A549 cells to apoptosis with 200 μg/mL concentration treated for 24 h. RT-PCR results suggested that GCP-1 and GCP-2 could be used as potential and effective apoptosis inducers on A549 cells by increasing BAX, CASPASE-3, CASPASE-9, TIMP-1, TIMP-2 expression and decreasing BCL-2 expression. This research provided an innovative approach to using a byproduct of biodiesel production (crude glycerol) to produce polysaccharides of potential medicinal benefit. View Full-Text
Keywords: Chaetomium globosum CGMCC 6882; glycerol; crude glycerol; polysaccharide; anticancer activity Chaetomium globosum CGMCC 6882; glycerol; crude glycerol; polysaccharide; anticancer activity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Wang, Z.; Chen, P.; Tao, N.; Zhang, H.; Li, R.; Zhan, X.; Wang, F.; Shen, Y. Anticancer Activity of Polysaccharides Produced from Glycerol and Crude Glycerol by an Endophytic Fungus Chaetomium globosum CGMCC 6882 on Human Lung Cancer A549 Cells. Biomolecules 2018, 8, 171.

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