FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis

Khan, Firdos Alam; Akhtar, Sultan; Almofty, Sarah Ameen; Almohazey, Dana; Alomari, Munthar

doi:10.3390/biom8020032

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FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis

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Department of Stem Cell Biology, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, Post Box No. 1982, Dammam 31441, Saudi Arabia

Department of Biophysics, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, Post Box No. 1982, Dammam 31441, Saudi Arabia

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Biomolecules 2018, 8(2), 32; https://doi.org/10.3390/biom8020032

Received: 9 April 2018 / Revised: 13 May 2018 / Accepted: 15 May 2018 / Published: 23 May 2018

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Abstract

Currently, breast cancer treatment mostly revolves around radiation therapy and surgical interventions, but often these treatments do not provide satisfactory relief to the patients and cause unmanageable side-effects. Nanomaterials show promising results in treating cancer cells and have many advantages such as high biocompatibility, bioavailability and effective therapeutic capabilities. Interestingly, fluorescent magnetic nanoparticles have been used in many biological and diagnostic applications, but there is no report of use of fluorescent magnetic submicronic polymer nanoparticles (FMSP-nanoparticles) in the treatment of human breast cancer cells. In the present study, we tested the effect of FMSP-nanoparticles on human breast cancer cells (MCF-7). We tested different concentrations (1.25, 12.5 and 50 µg/mL) of FMSP-nanoparticles in MCF-7 cells and evaluated the nanoparticles response morphometrically. Our results revealed that FMSP-nanoparticles produced a concentration dependent effect on the cancer cells, a dose of 1.25 µg/mL produced no significant effect on the cancer cell morphology and cell death, whereas dosages of 12.5 and 50 µg/mL resulted in significant nuclear augmentation, disintegration, chromatic condensation followed by dose dependent cell death. Our results demonstrate that FMSP-nanoparticles induce cell death in MCF-7 cells and may be a potential anti-cancer agent for breast cancer treatment. View Full-Text

Keywords: fluorescent magnetic submicronic polymer nanoparticles; human breast cancer; MCF-7 cell line; anticancer; cytotoxicity; in vitro cell culture

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MDPI and ACS Style

Khan, F.A.; Akhtar, S.; Almofty, S.A.; Almohazey, D.; Alomari, M. FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis. Biomolecules 2018, 8, 32. https://doi.org/10.3390/biom8020032

AMA Style

Khan FA, Akhtar S, Almofty SA, Almohazey D, Alomari M. FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis. Biomolecules. 2018; 8(2):32. https://doi.org/10.3390/biom8020032

Chicago/Turabian Style

Khan, Firdos A., Sultan Akhtar, Sarah A. Almofty, Dana Almohazey, and Munthar Alomari. 2018. "FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis" Biomolecules 8, no. 2: 32. https://doi.org/10.3390/biom8020032

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FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis

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