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Biomolecules
Volume 13
Issue 2
10.3390/biom13020296
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Article

Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation

by
Anton De Spiegeleer
1,2,3,4,
Amélie Descamps
1,3,
Srinath Govindarajan
4,5,
Julie Coudenys
1,4,5,
Kevin Van der borght
3,
Hannah Hirmz
3,
Nele Van Den Noortgate
1,2,
Dirk Elewaut
1,4,5,
Bart De Spiegeleer
1,3 and
Evelien Wynendaele
1,3,*
1
Translational Research in Immunosenescence, Gerontology and Geriatrics (TRIGG) Group, Ghent University, 9000 Ghent, Belgium
2
Department of Geriatrics, Faculty of Medicine and Health Sciences, Ghent University Hospital, 9000 Ghent, Belgium
3
Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, Belgium
4
VIB Inflammation Research Center, Unit for Molecular Immunology and Inflammation, Ghent University, 9000 Ghent, Belgium
5
Department of Rheumatology, Faculty of Medicine and Health Sciences, Ghent University Hospital, 9000 Ghent, Belgium
*
Author to whom correspondence should be addressed.
Biomolecules 2023, 13(2), 296; https://doi.org/10.3390/biom13020296
Submission received: 21 December 2022 / Revised: 21 January 2023 / Accepted: 1 February 2023 / Published: 4 February 2023
(This article belongs to the Special Issue Nature Inspired Peptides in Medical Sciences)
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Abstract

Quorum-sensing peptides (QSPs) are bacterial peptides traditionally considered only as inter-bacterial communication molecules. Recently, their involvement in microbiome–host interactions influencing host diseases such as cancer and sarcopenia were explored. However, it is still unknown to what extent these peptides have the potential to modulate the immune system. In this proof-of-concept study, we screened 89 QSPs for their potential to induce IL-6 and TNFα in murine splenocytes and J774 macrophages. Confirmatory experiments on the positive screening-hits were conducted using murine splenocytes and human PBMCs of different ages. Finally, to investigate the biological relevance of immunomodulatory QSPs, we analysed plasma in a human cohort for the presence of the immunomodulatory QSP Q010. To do this, we used a newly developed UHPLC-MS/MS method. Our findings indicated that specific QSPs activate immune cells in vitro, with Q007, Q010, Q017 and Q212 being the top four screening hits. Q007 and Q010 were affirmed in subsequent confirmatory experiments using murine splenocytes and human PBMCs. Finally, Q010 was detected in human plasma, demonstrating for the first time the presence of an immunomodulatory QSP in human circulation. In conclusion, our data are the first evidence indicating the potential of biologically relevant quorum-sensing peptides to modulate the immune system.
Keywords: quorum-sensing peptides; microbiome; immune cells; J774; splenocytes; PBMC quorum-sensing peptides; microbiome; immune cells; J774; splenocytes; PBMC

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MDPI and ACS Style

De Spiegeleer, A.; Descamps, A.; Govindarajan, S.; Coudenys, J.; Van der borght, K.; Hirmz, H.; Van Den Noortgate, N.; Elewaut, D.; De Spiegeleer, B.; Wynendaele, E. Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation. Biomolecules 2023, 13, 296. https://doi.org/10.3390/biom13020296

AMA Style

De Spiegeleer A, Descamps A, Govindarajan S, Coudenys J, Van der borght K, Hirmz H, Van Den Noortgate N, Elewaut D, De Spiegeleer B, Wynendaele E. Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation. Biomolecules. 2023; 13(2):296. https://doi.org/10.3390/biom13020296

Chicago/Turabian Style

De Spiegeleer, Anton, Amélie Descamps, Srinath Govindarajan, Julie Coudenys, Kevin Van der borght, Hannah Hirmz, Nele Van Den Noortgate, Dirk Elewaut, Bart De Spiegeleer, and Evelien Wynendaele. 2023. "Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation" Biomolecules 13, no. 2: 296. https://doi.org/10.3390/biom13020296

APA Style

De Spiegeleer, A., Descamps, A., Govindarajan, S., Coudenys, J., Van der borght, K., Hirmz, H., Van Den Noortgate, N., Elewaut, D., De Spiegeleer, B., & Wynendaele, E. (2023). Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation. Biomolecules, 13(2), 296. https://doi.org/10.3390/biom13020296

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