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Article

A Homozygous Dab1−/− Is a Potential Novel Cause of Autosomal Recessive Congenital Anomalies of the Mice Kidney and Urinary Tract

1
Department of Anatomy, Histology and Embryology, University of Split School of Medicine, 21000 Split, Croatia
2
Department of Medical Genetics, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and Herzegovina
3
Division of Anatomy, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi 981-8558, Japan
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Department of Anatomy, Shiga University of Medical Science, Ötsu 520-2192, Japan
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Department of Pathology, University Hospital of Split, 21000 Split, Croatia
*
Author to whom correspondence should be addressed.
Academic Editor: Theodoros Eleftheriadis
Biomolecules 2021, 11(4), 609; https://doi.org/10.3390/biom11040609
Received: 19 March 2021 / Revised: 14 April 2021 / Accepted: 19 April 2021 / Published: 20 April 2021
(This article belongs to the Special Issue Biomolecules in Development and Diseases of Urogenital system)
This study aimed to explore morphology changes in the kidneys of Dab1−/− (yotari) mice, as well as expression patterns of reelin, NOTCH2, LC3B, and cleaved caspase3 (CASP3) proteins, as potential determinants of normal kidney formation and function. We assumed that Dab1 functional inactivation may cause disorder in a wide spectrum of congenital anomalies of the kidney and urinary tract (CAKUT). Animals were sacrificed at postnatal days P4, P11, and P14. Paraffin-embedded kidney tissues were sectioned and analyzed by immunohistochemistry using specific antibodies. Kidney specimens were examined by bright-field, fluorescence, and electron microscopy. Data were analyzed by two-way ANOVA and t-tests. We noticed that yotari kidneys were smaller in size with a reduced diameter of nephron segments and thinner cortex. TEM microphotographs revealed foot process effacement in the glomeruli (G) of yotari mice, whereas aberrations in the structure of proximal convoluted tubules (PCT) and distal convoluted tubules (DCT) were not observed. A significant increase in reelin expression, NOTCH2, LC3B and cleaved CASP3 proteins was observed in the glomeruli of yotari mice. Renal hypoplasia in conjunction with foot process effacement and elevation in the expression of examined proteins in the glomeruli revealed CAKUT phenotype and loss of functional kidney tissue of yotari. View Full-Text
Keywords: yotari; kidney function; postnatal kidney development; immunofluorescence staining; transmission electron microscopy yotari; kidney function; postnatal kidney development; immunofluorescence staining; transmission electron microscopy
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MDPI and ACS Style

Racetin, A.; Filipović, N.; Lozić, M.; Ogata, M.; Gudelj Ensor, L.; Kelam, N.; Kovačević, P.; Watanabe, K.; Katsuyama, Y.; Saraga-Babić, M.; Glavina Durdov, M.; Vukojević, K. A Homozygous Dab1−/− Is a Potential Novel Cause of Autosomal Recessive Congenital Anomalies of the Mice Kidney and Urinary Tract. Biomolecules 2021, 11, 609. https://doi.org/10.3390/biom11040609

AMA Style

Racetin A, Filipović N, Lozić M, Ogata M, Gudelj Ensor L, Kelam N, Kovačević P, Watanabe K, Katsuyama Y, Saraga-Babić M, Glavina Durdov M, Vukojević K. A Homozygous Dab1−/− Is a Potential Novel Cause of Autosomal Recessive Congenital Anomalies of the Mice Kidney and Urinary Tract. Biomolecules. 2021; 11(4):609. https://doi.org/10.3390/biom11040609

Chicago/Turabian Style

Racetin, Anita, Natalija Filipović, Mirela Lozić, Masaki Ogata, Larissa Gudelj Ensor, Nela Kelam, Petra Kovačević, Koichiro Watanabe, Yu Katsuyama, Mirna Saraga-Babić, Merica Glavina Durdov, and Katarina Vukojević. 2021. "A Homozygous Dab1−/− Is a Potential Novel Cause of Autosomal Recessive Congenital Anomalies of the Mice Kidney and Urinary Tract" Biomolecules 11, no. 4: 609. https://doi.org/10.3390/biom11040609

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