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Article

The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers

1
Department of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, Germany
2
School of (EAST) Engineering, Arts, Science & Technology, University of Suffolk, Ipswich IP 41QJ, UK
3
Clinic for Hematology, Cell Therapy and Hemostaseology, University Hospital Leipzig, 04103 Leipzig, Germany
4
Polyclinic for Dental Prosthetics and Material Sciences, University Hospital Leipzig, 04103 Leipzig, Germany
5
Medical Faculty, Institute for Clinical Immunology, University Hospital Leipzig, 04103 Leipzig, Germany
6
Department of Immunology, Institute for Biological Research ‘Sinisa Stankovic’ (IBISS)-National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia
7
Clinic for Dermatology, Venereology and Allergology, University Hospital Leipzig, 04103 Leipzig, Germany
*
Author to whom correspondence should be addressed.
Bernd Lethaus and Vuk Savkovic contributed equally to this work.
Academic Editor: Vladimir N. Uversky
Biomolecules 2021, 11(2), 154; https://doi.org/10.3390/biom11020154
Received: 13 December 2020 / Revised: 15 January 2021 / Accepted: 19 January 2021 / Published: 25 January 2021
(This article belongs to the Collection Mesenchymal Stem Cell Fate and Potential Therapy)
Hair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which require human scalp tissue ex vivo. Non-invasive sampling has been standardized by means of the plucking procedure, enabling to reproducibly obtain the mid-ORS part. The mid-ORS shows potential for giving rise to multiple stem cell populations in vitro. To demonstrate the phenotypic features of distal, middle, and proximal ORS parts, gene and protein expression profiles were studied in physically separated portions. The mid-part of the ORS showed a comparable or higher NGFR, nestin/NES, CD34, CD73, CD44, CD133, CK5, PAX3, MITF, and PMEL expression on both protein and gene levels, when compared to the distal ORS part. Distinct subpopulations of cells exhibiting small and round morphology were characterized with flow cytometry as simultaneously expressing CD73/CD271, CD49f/CD105, nestin, and not CK10. Potentially, these distinct subpopulations can give rise to cultured neuroectodermal and mesenchymal stem cell populations in vitro. In conclusion, the mid part of the ORS holds the potential for yielding multiple stem cells, in particular mesenchymal stem cells. View Full-Text
Keywords: hair follicle; outer root sheath; mesenchymal stem cells; neuroectodermal cells; non-invasive sampling; multipotency; in vitro cultivation hair follicle; outer root sheath; mesenchymal stem cells; neuroectodermal cells; non-invasive sampling; multipotency; in vitro cultivation
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MDPI and ACS Style

Li, H.; Masieri, F.F.; Schneider, M.; Bartella, A.; Gaus, S.; Hahnel, S.; Zimmerer, R.; Sack, U.; Maksimovic-Ivanic, D.; Mijatovic, S.; Simon, J.-C.; Lethaus, B.; Savkovic, V. The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers. Biomolecules 2021, 11, 154. https://doi.org/10.3390/biom11020154

AMA Style

Li H, Masieri FF, Schneider M, Bartella A, Gaus S, Hahnel S, Zimmerer R, Sack U, Maksimovic-Ivanic D, Mijatovic S, Simon J-C, Lethaus B, Savkovic V. The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers. Biomolecules. 2021; 11(2):154. https://doi.org/10.3390/biom11020154

Chicago/Turabian Style

Li, Hanluo, Federica F. Masieri, Marie Schneider, Alexander Bartella, Sebastian Gaus, Sebastian Hahnel, Rüdiger Zimmerer, Ulrich Sack, Danijela Maksimovic-Ivanic, Sanja Mijatovic, Jan-Christoph Simon, Bernd Lethaus, and Vuk Savkovic. 2021. "The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers" Biomolecules 11, no. 2: 154. https://doi.org/10.3390/biom11020154

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