Next Article in Journal
Caveolae and Lipid Rafts in Endothelium: Valuable Organelles for Multiple Functions
Previous Article in Journal
Study of Biomolecular Interactions of Mitochondrial Proteins Related to Alzheimer’s Disease: Toward Multi-Interaction Biomolecular Processes
Open AccessArticle

The Influence of Nasturtium officinale R. Br. Agar and Agitated Microshoot Culture Media on Glucosinolate and Phenolic Acid Production, and Antioxidant Activity

1
Chair and Department of Pharmaceutical Botany, Faculty of Pharmacy, Medical College, Jagiellonian University, ul. Medyczna 9, 30-688 Kraków, Poland
2
Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, ul. Niezapominajek 21, 30-239 Kraków, Poland
3
Department of Medicinal Chemistry, Faculty of Pharmacy with Division of Medical Analytics, Medical University of Lublin, ul. Chodźki 4a, 20-093 Lublin, Poland
4
Department of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(9), 1216; https://doi.org/10.3390/biom10091216
Received: 22 July 2020 / Revised: 16 August 2020 / Accepted: 18 August 2020 / Published: 21 August 2020
This paper presents an optimization of conditions for microshoot cultures of Nasturtium officinale R. Br. (watercress). Variants of the Murashige and Skoog (MS) medium containing different plant growth regulators (PGRs): cytokinins—BA (6-benzyladenine), 2iP (6-γ,γ-dimethylallylaminopurine), KIN (kinetin), Zea (zeatin), and auxins—IAA (3-indoleacetic acid), IBA (indole-3-butyric acid), 2,4-d (2,4-dichlorophenoxyacetic acid), IPA (indole-3-pyruvic acid), NAA (naphthalene-1-acetic acid), total 27 MS variants, were tested in agar and agitated cultures. Growth cycles were tested for 10, 20, or 30 days in the agar cultures, and 10 or 20 days in the agitated cultures. Glucosinolate and phenolic acid production, total phenolic content and antioxidant potential were evaluated. The total amounts of glucosinolates ranged from 100.23 to 194.77 mg/100 g dry weight of biomass (DW) in agar cultures, and from 78.09 to 182.80 mg/100 g DW in agitated cultures. The total phenolic acid content varied from 15.89 to 237.52 mg/100 g DW for the agar cultures, and from 70.80 to 236.74 mg/100 g DW for the agitated cultures. Extracts of the cultured biomass contained higher total amounts of phenolic acids, lower total amounts of glucosinolates, a higher total phenolic content and similar antioxidant potentials compared to plant material. The analyses performed confirmed for the first time the explicit influence on secondary metabolite production and on the antioxidant potential. The significance was statistically estimated in a complex manner. View Full-Text
Keywords: in vitro cultures; plant growth regulators; phenolic acids; glucosinolates; antioxidant potential in vitro cultures; plant growth regulators; phenolic acids; glucosinolates; antioxidant potential
Show Figures

Graphical abstract

MDPI and ACS Style

Klimek-Szczykutowicz, M.; Szopa, A.; Dziurka, M.; Komsta, Ł.; Tomczyk, M.; Ekiert, H. The Influence of Nasturtium officinale R. Br. Agar and Agitated Microshoot Culture Media on Glucosinolate and Phenolic Acid Production, and Antioxidant Activity. Biomolecules 2020, 10, 1216.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop