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Open AccessArticle

Tumoricidal and Bactericidal Properties of ZnONPs Synthesized Using Cassia auriculata Leaf Extract

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Department of Sciences, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru, Karnataka–570 026, India
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Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka–570 015, India
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Department of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia
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Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Qassim 51431, Saudi Arabia
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National Center for Biotechnology, Life Science and Environmental Research Institute, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 12354, Saudi Arabia
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National Center for Biotechnology, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 12354, Saudi Arabia
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Department of Biotechnology, Davangere University, Shivagangotri, Karnataka 577 007, India
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Department of Studies in Botany, University of Mysore, Manasagangotri, Mysuru 570 006, Karnataka, India
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Department of Chemistry, Bangalore Institute of Technology, K.R. Road, V V Puram, Karnataka, Bangalore 560 004, India
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(7), 982; https://doi.org/10.3390/biom10070982
Received: 2 June 2020 / Revised: 25 June 2020 / Accepted: 25 June 2020 / Published: 30 June 2020
In this work, we aimed to synthesize zinc oxide nanoparticles (ZnONPs) using an aqueous extract of Cassia auriculata leaves (CAE) at room temperature without the provision of additional surfactants or capping agents. The formation of as-obtained ZnONPs was analyzed by UV–visible (ultraviolet) absorption and emission spectroscopy, X-ray photoemission spectroscopy (XPS), X-ray diffraction analysis (XRD), energy dispersive X-ray diffraction (EDX), thermogravimetric analysis/differential thermal analysis (TGA-DTA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), and selected area electron diffraction (SAED). The XRD results reflect the wurtzite structure of as-prepared ZnONPs, which produced diffraction patterns showing hexagonal phases. The SEM images indicate that the morphology of as-prepared ZnONPs is composed of hexagonal nanostructures with an average diameter of 20 nm. The HR-TEM result shows that the inter-planar distance between two lattice fringes is 0.260 nm, which coincides with the distance between the adjacent (d-spacing) of the (002) lattice plane of ZnO. The fluorescence emission spectrum of ZnONPs dispersed in ethanol shows an emission maximum at 569 nm, revealing the semiconductor nature of ZnO. As-obtained ZnONPs enhanced the tumoricidal property of CAE in MCF-7 breast cancer cells without significant inhibition of normal human breast cells, MCF-12A. Furthermore, we have studied the antibacterial effects of ZnONPs, which showed direct cell surface contact, resulting in the disturbance of bacterial cell integrity.
Keywords: zinc oxide nanoparticles (ZnONPs); Cassia auriculata; anticancer property; X-ray photoemission spectroscopy (XRD) pattern zinc oxide nanoparticles (ZnONPs); Cassia auriculata; anticancer property; X-ray photoemission spectroscopy (XRD) pattern
MDPI and ACS Style

Prasad, K.S.; Prasad, S.K.; Ansari, M.A.; Alzohairy, M.A.; Alomary, M.N.; AlYahya, S.; Srinivasa, C.; Murali, M.; Ankegowda, V.M.; Shivamallu, C. Tumoricidal and Bactericidal Properties of ZnONPs Synthesized Using Cassia auriculata Leaf Extract. Biomolecules 2020, 10, 982.

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