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Possible Mechanisms of Eosinophil Accumulation in Eosinophilic Pneumonia
Article

Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis

Department of Otorhinolaryngology Head & Neck Surgery, Faculty of Medical Sciences, University of Fukui, Fukui 910-1193, Japan
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Biomolecules 2020, 10(11), 1568; https://doi.org/10.3390/biom10111568
Received: 25 October 2020 / Revised: 14 November 2020 / Accepted: 16 November 2020 / Published: 18 November 2020
(This article belongs to the Special Issue Eosinophilic Inflammation)
Background: The pathological features of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) tissues include an eosinophilic infiltration pattern (eosinophilic CRS (ECRS)) or a less eosinophilic pattern (non-ECRS). Recently, it has been suggested that 15-lipoxygenase 1 (15-LOX-1) may have significant roles in allergic disease; however, the significance of 15-LOX-1 in CRS is not well understood. The objective of this study was to demonstrate the expression of 15-LOX-1 in CRS. Methods: The mRNA expression levels of 15-LOX-1 and periostin in nasal tissues were measured by quantitative real-time polymerase chain reaction. We also performed an immunofluorescence study of nasal tissues. Cells of the Eol-1 eosinophilic leukemic cell line were stimulated with interleukin-33 to test the induction of 15-LOX-1. Results: The expression level of 15-LOX-1 mRNA in nasal polyps (NPs) was significantly higher in ECRS patients than in non-ECRS patients. The immunofluorescence study revealed that both airway epithelial cells and eosinophils in NPs expressed 15-LOX-1. A significant correlation was seen between the number of eosinophils and the mRNA expression levels of 15-LOX-1 and periostin in nasal polyps. Moreover, interleukin-33 enhanced 15-LOX-1 expression in Eol-1 cells. Conclusions: 15-LOX-1 was shown to be a significant molecule that facilitates eosinophilic inflammation in ECRS. View Full-Text
Keywords: CRS; 15-LOX-1; eosinophils; periostin; IL-33 CRS; 15-LOX-1; eosinophils; periostin; IL-33
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MDPI and ACS Style

Imoto, Y.; Takabayashi, T.; Sakashita, M.; Kato, Y.; Yoshida, K.; Kidoguchi, M.; Koyama, K.; Adachi, N.; Kimura, Y.; Ogi, K.; Ito, Y.; Kanno, M.; Okamoto, M.; Narita, N.; Fujieda, S. Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis. Biomolecules 2020, 10, 1568. https://doi.org/10.3390/biom10111568

AMA Style

Imoto Y, Takabayashi T, Sakashita M, Kato Y, Yoshida K, Kidoguchi M, Koyama K, Adachi N, Kimura Y, Ogi K, Ito Y, Kanno M, Okamoto M, Narita N, Fujieda S. Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis. Biomolecules. 2020; 10(11):1568. https://doi.org/10.3390/biom10111568

Chicago/Turabian Style

Imoto, Yoshimasa, Tetsuji Takabayashi, Masafumi Sakashita, Yukinori Kato, Kanako Yoshida, Masanori Kidoguchi, Keisuke Koyama, Naoto Adachi, Yukihiro Kimura, Kazuhiro Ogi, Yumi Ito, Masafumi Kanno, Masayuki Okamoto, Norihiko Narita, and Shigeharu Fujieda. 2020. "Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis" Biomolecules 10, no. 11: 1568. https://doi.org/10.3390/biom10111568

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